IL-25 and IL-25 receptor expression on eosinophils from subjects with allergic asthma
| Autor: | Steven G. Smith, Benny Dua, Wei Tang, Paul M. O'Byrne, Gail M. Gauvreau, Karen Howie, Sue Beaudin |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Adult
Hypersensitivity Immediate Male Receptor expression Immunology Gene Expression Neutrophil Activation Allergic inflammation Leukocyte Count Immunology and Allergy Medicine Humans CCL13 Receptor Interleukin 5 Asthma Receptors Interleukin-17 business.industry Interleukin-17 Case-control study Interleukin General Medicine Middle Aged medicine.disease respiratory tract diseases Eosinophils Protein Subunits Case-Control Studies Female business |
| Zdroj: | International archives of allergy and immunology. 163(1) |
| ISSN: | 1423-0097 |
| Popis: | Background: Allergic asthma is an inflammatory airway disease in which Th2 cytokines play an important role. Epithelial-derived interleukin (IL)-25 has been suggested to be important in the maintenance of Th2-type responses. The effects of IL-25 are mediated by the IL-25 receptor, composed of two subunits, IL-17RA and IL-17RB. Eosinophils are effector cells in allergic asthma. The role of IL-25 in eosinophil function is unknown. This study examined the expression of IL-25 and its receptor on eosinophils in allergic asthmatics compared to atopic nonasthmatics and normal controls. Methods: The expression of IL-25, IL-17RA and IL-17RB on eosinophils, and levels of plasma IL-25 were measured in 14 normal control subjects, 15 atopic nonasthmatics and 14 mild allergic asthmatics. Results: The expression of IL-17RB on eosinophils was significantly higher in allergic asthmatics (43.08%, range 33.96-59.98%) than in atopic nonasthmatics (11.98%, range 6.33-27.11%, p = 0.002) and normal controls (17.70%, range 10.97-38.9%, p = 0.01). IL-17RA expression was also significantly higher in the allergic asthmatic group. No differences were observed in the intracellular expression of IL-25. The concentration of IL-25 in plasma was significantly increased in the allergic asthmatics (145 ng/ml, range 64-290 ng/ml) when compared to the normal controls (21 ng/ml, range 0-116 ng/ml, p = 0.012), but not compared to atopic nonasthmatics. There was a significant negative correlation between FEV1 % predicted and the IL-25 level in the plasma (r = -0.443, p = 0.003). Conclusions: The increased IL-25 levels in plasma and the expression of IL-17RA and IL-17RB on eosinophils in allergic asthma patients suggests that IL-25 may activate eosinophils during allergic inflammation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|