Assessment of the inhibitory effects of different radiation qualities or chemotherapeutic agents on a human melanoma cell line
| Autor: | L. M. Valastro, Giacomo Cuttone, Ivan Petrović, Aleksandra Ristić-Fira, Giuseppe Privitera, Lela B. Korićanac |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Cell Survival
Dacarbazine HTB140 melanoma cells Biophysics Sulforhodamine B General Physics and Astronomy Antineoplastic Agents Apoptosis Cellular sensitivity Pharmacology Radiation Dosage Alkylating agents 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor medicine Relative biological effectiveness Humans Radiology Nuclear Medicine and imaging gamma-Rays Cytotoxicity Clonogenic assay Melanoma IC50 030304 developmental biology 0303 health sciences Dose-Response Relationship Drug business.industry Dose-Response Relationship Radiation General Medicine chemistry 030220 oncology & carcinogenesis Fotemustine Protons Growth inhibition Nuclear medicine business medicine.drug |
| Zdroj: | Physica Medica |
| ISSN: | 1120-1797 |
| Popis: | The correlation between time dependent viabilities, after applying two radiation qualities and two alkylating agents on HTB140 melanoma cells, has been studied. Irradiations were performed with gamma-rays and 62 MeV protons, close to the Bragg peak maximum, delivering doses of 8-24 Gy. Treatments with fotemustine (FM) and dacarbazine (DTIC) were carried out with concentrations of 0.05-2 mM. High radio-resistance of HTB140 cells revealed by a clonogenic assay was confirmed by microtetrasolium and sulforhodamine B, through the surviving fraction at 2 Gy (SF2), being 0.961-0.956 for gamma-rays and 0.931-0.887 for protons. A better efficiency of protons was illustrated by relative biological effectiveness at 2 Gy (RBE), ranging from 1.69 to 1.89. A kinetic study of concentration dependent cytotoxicity indicated that the best effect of the drugs, estimated as the concentration that produces 50% of growth inhibition (IC(50)), was obtained at 48 h, having values of 76 mu M for DTIC and 145 mu M for FM. The cytostatic ability of the drugs pointed out that the presence of DTIC at 24 h, compared to FM, was insufficient to produce an effect. Protons and FM demonstrated their pro apoptotic capacity. Cross-resistance between treatments applied to the HTB140 cells was observed, protons being the most efficient, while DTIC, FM and gamma-rays demonstrated a lower level of cell inactivation. (C) 2008 Associazione Italiana di Fisica Medica. Published by Elsevier Ltd. ALL rights reserved. |
| Databáze: | OpenAIRE |
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