Concentration of hepatic vitamins A and E in rats exposed to chlorpyrifos and/or enrofloxacin
Autor: | D. Barski, Spodniewska A |
---|---|
Rok vydání: | 2016 |
Předmět: |
Male
0301 basic medicine Vitamin Insecticides Antioxidant medicine.medical_treatment Group ii 010501 environmental sciences 01 natural sciences Lipid peroxidation Toxicology 03 medical and health sciences chemistry.chemical_compound Animal science medicine Enrofloxacin Animals Vitamin E Drug Interactions Rats Wistar Vitamin A 0105 earth and related environmental sciences General Veterinary Chemistry General Medicine Antioxidant vitamins Anti-Bacterial Agents Rats 030104 developmental biology Liver Chlorpyrifos Fluoroquinolones medicine.drug |
Zdroj: | Polish Journal of Veterinary Sciences. 19:371-378 |
ISSN: | 2300-2557 |
Popis: | The aim of the study was to determine the level of antioxidant vitamins A and E in the liver of rats exposed to chlorpyrifos and/or enrofloxacin. Chlorpyrifos (Group I) was administered at a dose of 0.04 LD50 (6 mg/kg b.w.) for 28 days, and enrofloxacin (Group II) at a dose of 5 mg/kg b.w. for 5 consecutive days. The animals of group III were given both of the mentioned above compounds at the same manner as groups I and II, but enrofloxacin was applied to rats for the last 5 days of chlorpyrifos exposure (i.e. on day 24, 25, 26, 27 and 28). Chlorpyrifos and enrofloxacin were administered to rats intragastrically via a gastric tube. The quantitative determination of vitamins was made by the HPLC method. The results of this study indicated a reduction in the hepatic concentrations of vitamins A and E, compared to the control, which sustained for the entire period of the experiment. The four-week administration of chlorpyrifos to rats resulted in a significant decrease of vitamins in the initial period of the experiment, i.e. up to 24 hours after exposure. For vitamin A the maximum drop was observed after 24 hours (19.24%) and for vitamin E after 6 hours (23.19%). Enrofloxacin caused a slight (3-9%) reduction in the level of the analysed vitamins. In the chlorpyrifos-enrofloxacin co-exposure group reduced vitamins A and E levels were also noted, but changes in this group were less pronounced in comparison to the animals intoxicated with chlorpyrifos only. The decrease in the antioxidant vitamin levels, particularly noticeable in the chlorpyrifos- and the chlorpyrifos combined with enrofloxacin-treated groups, may result not only from the increase in the concentration of free radicals, but also from the intensification of the secondary stages of lipid peroxidation. |
Databáze: | OpenAIRE |
Externí odkaz: |