Ultrasensitive, Label Free, Chemiresistive Nanobiosensor Using Multiwalled Carbon Nanotubes Embedded Electrospun SU-8 Nanofibers
| Autor: | Shiv Govind Singh, Matta Durga Prakash, Chandra Shekhar Sharma, Siva Rama Krishna Vanjari |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Materials science
Nanotechnology 02 engineering and technology Photoresist ultra sensitivity 010402 general chemistry lcsh:Chemical technology 01 natural sciences Biochemistry Article Analytical Chemistry lcsh:TP1-1185 Electrical and Electronic Engineering MWCNTs Instrumentation electrospinning Detection limit chemiresistive Multielectrode array 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics Electrospinning 0104 chemical sciences SU-8 nanobiosensors Nanofiber Electrode Surface modification 0210 nano-technology Biosensor |
| Zdroj: | Sensors (Basel, Switzerland) Sensors, Vol 16, Iss 9, p 1354 (2016) Sensors; Volume 16; Issue 9; Pages: 1354 |
| ISSN: | 1424-8220 |
| Popis: | This paper reports the synthesis and fabrication of aligned electrospun nanofibers derived out of multiwalled carbon nanotubes (MWCNTs) embedded SU-8 photoresist, which are targeted towards ultrasensitive biosensor applications. The ultrasensitivity (detection in the range of fg/mL) and the specificity of these biosensors were achieved by complementing the inherent advantages of MWCNTs such as high surface to volume ratio and excellent electrical and transduction properties with the ease of surface functionalization of SU-8. The electrospinning process was optimized to precisely align nanofibers in between two electrodes of a copper microelectrode array. MWCNTs not only enhance the conductivity of SU-8 nanofibers but also act as transduction elements. In this paper, MWCNTs were embedded way beyond the percolation threshold and the optimum percentage loading of MWCNTs for maximizing the conductivity of nanofibers was figured out experimentally. As a proof of concept, the detection of myoglobin, an important biomarker for on-set of Acute Myocardial Infection (AMI) has been demonstrated by functionalizing the nanofibers with anti-myoglobin antibodies and carrying out detection using a chemiresistive method. This simple and robust device yielded a detection limit of 6 fg/mL. |
| Databáze: | OpenAIRE |
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