Method for Malaria Diagnosis Based on Extractions of Samples Using Non-Invasive Techniques: An Opportunity for the Nursing Clinical Practice
| Autor: | Silvia Belinchón Lorenzo, J.C. Parejo, Fidel López-Espuela, María Zoraida Clavijo-Chamorro, Faustin Munyaruguru, Isabel Monroy, Adela Gómez-Luque, Luis Carlos Gómez-Nieto |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Health Toxicology and Mutagenesis Plasmodium falciparum 030231 tropical medicine lcsh:Medicine Noninvasive techniques Biology Real-Time Polymerase Chain Reaction Sensitivity and Specificity Article DNA sequencing Specimen Handling 03 medical and health sciences 0302 clinical medicine parasitic diseases medicine Animals Humans 030212 general & internal medicine Malaria Falciparum Rapid diagnostic test Blood Specimen Collection Diagnostic Tests Routine lcsh:R Public Health Environmental and Occupational Health DNA DNA Protozoan Leishmania biology.organism_classification medicine.disease Virology Malaria Diagnosis of malaria Real-time polymerase chain reaction Female Sample collection Hair |
| Zdroj: | International Journal of Environmental Research and Public Health Volume 17 Issue 15 International Journal of Environmental Research and Public Health, Vol 17, Iss 5551, p 5551 (2020) |
| ISSN: | 1660-4601 |
| DOI: | 10.3390/ijerph17155551 |
| Popis: | Malaria has been for millennia one of the best known and most destructive diseases affecting humans. Its high impact has aroused great interest for the development of new effective and reliable diagnostic techniques. Recently it has been recently published that hairs from mammal hosts are able to capture, hold and finally remove foreign DNA sequences of Leishmania parasites. The aim of this study was to check if Plasmodium falciparum (P. falciparum) DNA remains stable in blood samples deposited in Whatman paper after suffering different transport and storage conditions, and to compare the sensitivity of these results with those offered by thick a smear and Rapid Diagnostic Test, and besides to examine whether P. falciparum DNA would be detected and quantified by Real time quantitative PCR (qPCR) from hairs of people with different types of malaria. P. falciparum Histidine Repeat Protein II (pHRP-II) antigen detection and P. falciparum DNA were detected in 18 of 19 dry blood samples adhered to Whatman paper (94.74%), besides, Plasmodium DNA was also detected in seven out of 19 hair samples analyzed (36.84%), remaining stable until analysis for several months under the exposure to different environmental conditions. Although the sensitivity of PCR for the diagnosis of malaria in hair samples is not as high as blood analysis, the study of Plasmodium DNA presence in blood and hair could constitute a complementary tool with numerous advantages in sample collection, transport and storage. We suggest that the method could be also applied to medical, forensic and paleo-parasitological diagnosis, not only for malaria but also for searching many other pathogens in hair samples. |
| Databáze: | OpenAIRE |
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