Selection of protein-protein interactions of desired affinities with a bandpass circuit
| Autor: | Katherine E. Brechun, G. Andrew Woolley, Katja M. Arndt |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Population
Interaction strength Protein Engineering Article beta-Lactamases Protein–protein interaction 03 medical and health sciences Negative selection 0302 clinical medicine Band-pass filter Structural Biology ddc:570 Escherichia coli Protein Interaction Maps education Molecular Biology Institut für Biochemie und Biologie Selection (genetic algorithm) 030304 developmental biology Selection system 0303 health sciences education.field_of_study Chemistry Escherichia coli Proteins Affinities Biological system 030217 neurology & neurosurgery |
| Popis: | We have developed a genetic circuit in Escherichia coli that can be used to select for protein-protein interactions of different strengths by changing antibiotic concentrations in the media. The genetic circuit links protein-protein interaction strength to beta-lactamase activity while simultaneously imposing tuneable positive and negative selection pressure for beta-lactamase activity. Cells only survive if they express interacting proteins with affinities that fall within set high- and low-pass thresholds; i.e. the circuit therefore acts as a bandpass filter for protein-protein interactions. We show that the circuit can be used to recover protein-protein interactions of desired affinity from a mixed population with a range of affinities. The circuit can also be used to select for inhibitors of protein-protein interactions of defined strength. (C) 2018 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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