Interrogation of metabolic and oxygen states of tumors with fiber-based luminescence lifetime spectroscopy
| Autor: | Hauke Studier, Antje Neubauer, Vladislav Shcheslavskiy, Anton I. Pavlikov, Toshitada Yoshihara, Marina V. Shirmanova, Anna Orlova, Seiji Tobita, Daniil Shirokov, Wolfgang Becker, Elena V. Zagaynova, Maria M. Lukina |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Optical fiber Materials science Pyridines chemistry.chemical_element Iridium Oxygen law.invention 03 medical and health sciences Mice Nuclear magnetic resonance Optics law Cell Line Tumor Organometallic Compounds Animals Humans Spectroscopy Optical Fibers Multi-mode optical fiber 030102 biochemistry & molecular biology business.industry Fluorescence Atomic and Molecular Physics and Optics 030104 developmental biology Spectrometry Fluorescence chemistry Optoelectronics Luminescence Phosphorescence business Oxygen sensor NADP |
| Zdroj: | Optics letters. 42(4) |
| ISSN: | 1539-4794 |
| Popis: | The study of metabolic and oxygen states of cells in a tumor in vivo is crucial for understanding of the mechanisms responsible for tumor development and provides background for the relevant tumor's treatment. Here, we show that a specially designed implantable fiber-optic probe provides a promising tool for optical interrogation of metabolic and oxygen states of a tumor in vivo. In our experiments, the excitation light from a ps diode laser source is delivered to the sample through an exchangeable tip via a multimode fiber, and the emission light is transferred to the detector by another multimode fiber. Fluorescence lifetime of a nicotinamid adenine dinucleotide (NAD(P)H) and phosphorescence lifetime of an oxygen sensor based on an iridium (III) complex of enzothienylpyridine (BTPDM1) are explored both in model experiment in solutions and in living mice. |
| Databáze: | OpenAIRE |
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