Assessment of microbial activity in water based on hydrogen peroxide decomposition rates
| Autor: | Erik Arvin, Per Bovbjerg Pedersen, Lars-Flemming Pedersen, Paula Andrea Rojas-Tirado |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
chemistry.chemical_classification biology Chemistry 010604 marine biology & hydrobiology 04 agricultural and veterinary sciences Aquatic Science Plankton biology.organism_classification 01 natural sciences Decomposition 6. Clean water chemistry.chemical_compound Reaction rate constant Algae Environmental chemistry 040102 fisheries 0401 agriculture forestry and fisheries Organic matter Water quality Hydrogen peroxide Bacteria |
| Zdroj: | Aquacultural Engineering |
| ISSN: | 0144-8609 |
| DOI: | 10.1016/j.aquaeng.2019.01.001 |
| Popis: | Embargo until 23 January 2021 This study proposes a new and simple assay that allows rapid assessment of microbial activity in water samples. The assay consists of standardized hydrogen peroxide (H2O2) addition to a water sample and subsequent spectrophotometric determination of H2O2 reduction over time. The H2O2 decomposition rate constant reflects the level of enzymatic activity from planktonic and particle-associated bacteria as well as algae and protozoans. The proof of concept was verified on water samples from recirculating aquaculture systems (RAS), showing that the vast majority of H2O2 decomposition was related to microbial activity. Only 3% of the total H2O2 decomposition was related to abiotic processes when 0.20 μm sterile filtered RAS water was compared with unfiltered RAS water. Planktonic bacteria (size range 0.20–1.6 μm) accounted for 16% of H2O2 decomposition, while bacterial aggregates, particle-associated bacteria and microbiota above 1.6 μm were responsible for the remaining 81%. H2O2 decomposition rate constants were positively correlated to BOD5 (r = 0.893; p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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