Biochemical aspects of immunotoxin preparation
| Autor: | F.K. Jansen, O. Gros, H. Vidal, P. Gros |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Chemical Phenomena
Protein subunit Immunology Ricin medicine.disease_cause Antibodies Coupling reaction Mice chemistry.chemical_compound Immunotoxin medicine Disulfide bonding Animals Immunology and Allergy Toxins Biological biology Chemistry Toxin Antibodies Monoclonal Chemical modification Molecular Weight Kinetics Cross-Linking Reagents Biochemistry Immunoglobulin G biology.protein Immunotherapy Antibody |
| Zdroj: | Journal of Immunological Methods. 81:283-297 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(85)90213-3 |
| Popis: | The preparation of immunotoxins, hybrid proteins formed by disulfide bonding an antibody and the A-chain of ricin, has been studied in detail. Optimal conditions, both for the modification of the antibody and the coupling reaction between the modified antibodies and the toxin subunit, have been determined. Conditions of time, temperature and stoichiometry studied suggested 2 protocols for each of the 2 steps of this preparation. Purification and analysis of the physiochemical and biochemical properties of the products yielded well-characterized agents, likely to be of value in clinical studies. |
| Databáze: | OpenAIRE |
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