Transcriptomics Analysis Indicates Trifarotene Reverses Acne-Related Gene Expression Changes
Autor: | Valérie Julia, Jayendra Kumar Krishnaswamy, Rajeev Chavda, Amir Khammari, S. Blanchet‐Réthoré, Brigitte Dréno |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Medicine (General)
Inflammation trifarotene Transcriptome Lesion Pathogenesis transcriptomics R5-920 cellular expression Gene expression Medicine Acne pathophysiology Original Research business.industry General Medicine Retinoic acid receptor gamma bioinformatics medicine.disease Gene expression profiling acne (acne vulgaris) Cancer research gene expression medicine.symptom business |
Zdroj: | Frontiers in Medicine Frontiers in Medicine, Vol 8 (2021) |
ISSN: | 2296-858X |
Popis: | Background and Objectives: Trifarotene is a topical retinoid selective for retinoic acid receptor gamma that was recently approved for treatment of acne vulgaris. We performed a gene expression analysis to identify the molecular and cellular impact of trifarotene treatment on acne papules.Methods: In this open-label prospective study, subjects with moderate inflammatory acne of the back were treated with trifarotene 0.005% or vehicle cream on dedicated areas for 27 days, and 4 biopsies were collected from each subject (1 from skin without a visible acne lesion and three at the site of an acne papule: one baseline, one after vehicle treatment, and one after trifarotene treatment). Large scale gene expression profiling of the biopsies was performed using Affymetrix technology, treatment-specific gene expression profiles were generated using statistical modeling, and pathway analysis was performed. Using single-cell RNAseq data, in silico deconvolution of transcriptomics data was performed to identify cellular signatures.Results: We discovered a unique set of 67 genes modulated by trifarotene that are primarily involved in cellular migration, inflammation, and extracellular matrix reorganization. Changes in cellular expression were similar in both trifarotene-treated and spontaneously-resolving lesions. However, only trifarotene treatment impacted SPP1+ macrophages, a subset of highly proliferative macrophages recently identified in fibrotic tissue.Conclusions: These results show that trifarotene has a novel action in acne treatment by affecting epidermal and immune components of acne pathogenesis. |
Databáze: | OpenAIRE |
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