Gut Microbiome and Metabolome Profiles Associated with High-Fat Diet in Mice
| Autor: | Sun-Jae Kwon, Ju-Yeon Pyo, Eun-Ju Kim, Kwang-Moon Cho, Hyunwoo Kim, Dae-Hun Park, Seung-Ho Seo, Seong-Eun Park, Jeong-Sang Kim, Hong-Seok Son, Jae-Kwon Jo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
obesity Firmicutes Endocrinology Diabetes and Metabolism Metabolite metabolite 030209 endocrinology & metabolism Gut flora Microbiology Biochemistry Article Serine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Metabolomics Lactobacillus medicine Metabolome Molecular Biology biology gut microbiota fungi food and beverages medicine.disease biology.organism_classification QR1-502 030104 developmental biology high-fat diet chemistry Dysbiosis |
| Zdroj: | Metabolites Volume 11 Issue 8 Metabolites, Vol 11, Iss 482, p 482 (2021) |
| ISSN: | 2218-1989 |
| DOI: | 10.3390/metabo11080482 |
| Popis: | Obesity can be caused by microbes producing metabolites it is thus important to determine the correlation between gut microbes and metabolites. This study aimed to identify gut microbiota-metabolomic signatures that change with a high-fat diet and understand the underlying mechanisms. To investigate the profiles of the gut microbiota and metabolites that changed after a 60% fat diet for 8 weeks, 16S rRNA gene amplicon sequencing and gas chromatography-mass spectrometry (GC-MS)-based metabolomic analyses were performed. Mice belonging to the HFD group showed a significant decrease in the relative abundance of Bacteroidetes but an increase in the relative abundance of Firmicutes compared to the control group. The relative abundance of Firmicutes, such as Lactococcus, Blautia, Lachnoclostridium, Oscillibacter, Ruminiclostridium, Harryflintia, Lactobacillus, Oscillospira, and Erysipelatoclostridium, was significantly higher in the HFD group than in the control group. The increased relative abundance of Firmicutes in the HFD group was positively correlated with fecal ribose, hypoxanthine, fructose, glycolic acid, ornithine, serum inositol, tyrosine, and glycine. Metabolic pathways affected by a high fat diet on serum were involved in aminoacyl-tRNA biosynthesis, glycine, serine and threonine metabolism, cysteine and methionine metabolism, glyoxylate and dicarboxylate metabolism, and phenylalanine, tyrosine, and trypto-phan biosynthesis. This study provides insight into the dysbiosis of gut microbiota and metabolites altered by HFD and may help to understand the mechanisms underlying obesity mediated by gut microbiota. |
| Databáze: | OpenAIRE |
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