Hybrid magnetic nanoparticle/nanogold clusters and their distance-dependent metal-enhanced fluorescence effect via DNA hybridization
| Autor: | Xue-fan Gu, Yong-li Yan, Lingze Zhang, Yongchun Liu, Daocheng Wu, Yan Li, Youshen Wu |
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| Rok vydání: | 2014 |
| Předmět: |
Absorption spectroscopy
Iron Analytical chemistry Metal Nanoparticles Nanoparticle Fluorescence Magnetics chemistry.chemical_compound Nucleic acid thermodynamics Microscopy Electron Transmission Microscopy Electrochemistry Nanotechnology General Materials Science Particle Size Magnetite Nanoparticles Serum Albumin Photons DNA–DNA hybridization Temperature Nucleic Acid Hybridization DNA Silanes Magnetic Fields Spectrometry Fluorescence chemistry Magnetic nanoparticles Spectrophotometry Ultraviolet Gold Fluorescein-5-isothiocyanate |
| Zdroj: | Nanoscale. 6:8681-8693 |
| ISSN: | 2040-3372 2040-3364 |
| Popis: | To improve the metal-enhanced fluorescence (MEF) effect of nanogolds (AuNPs) and accurately detect specific DNA sequences via DNA hybridization, novel hybrid magnetic nanoparticles/nanogold clusters (HMNCs) were designed based on finite-difference time-domain simulation results and prepared by using Fe3O4 and nanogolds. The nanogolds outside the HMNC were then conjugated with thiol-terminated DNA molecules, thus DNA modified-HMNCs (DNA-HMNCs) were obtained. The size distributions of these nanostructures were measured by a Malvern size analyzer, and their morphology was observed via transmission electron microscopy (TEM). The ultraviolet (UV)-visible (vis) absorption spectra of the samples were recorded with a UV-2600 spectrophotometer. Fluorescence spectra and the MEF effect were recorded using a spectrophotofluorometer, and lifetimes were determined using a time-correlated single photon counting apparatus. The prepared HMNCs were stable in aqueous solutions and had an average diameter of 87 ± 3.2 nm, with six to eight AuNPs around a single Fe3O4 nanoparticle. Fluorescein isothiocyanate (FITC) tagged DNA-HMNC conjugates exhibited a significant MEF effect and could accurately detect specific DNA sequences after DNA hybridization. This result indicates their various potential applications in sensors and biomedical fields. |
| Databáze: | OpenAIRE |
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