Violacein induces cell death by triggering mitochondrial membrane hyperpolarization in vitro
| Autor: | Angélica Maria de Sousa Leal, Tatjana Keesen de Souza Lima, Jana Dara Freires de Queiroz, Lucymara Fassarella Agnez-Lima, Silvia Regina Batistuzzo de Medeiros |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Microbiology (medical)
Programmed cell death Indoles Cell Survival Cytotoxicity CHO Cells Biology medicine.disease_cause Microbiology Cell Line HeLa Cricetulus medicine Animals Humans Inner mitochondrial membrane Membrane potential chemistry.chemical_classification Membrane Potential Mitochondrial Reactive oxygen species Cell Death biology.organism_classification Chromobacterium violaceum Cell biology Oxidative Stress chemistry Cell culture Mitochondrial membrane potential Reactive Oxygen Species Oxidative stress Research Article Violacein HeLa Cells |
| Zdroj: | BMC Microbiology |
| ISSN: | 1471-2180 |
| Popis: | Background Violacein is a purple pigment from Chromobacterium violaceum that possesses diverse biological and pharmacological properties. Among these, pro-oxidant and antioxidant activities have been suggested. However, the cytotoxic mechanisms induced by violacein are poorly understood and the improvement in knowledge regarding these cell death mechanisms will be useful to develop new therapeutic approaches. Considering this, in our work, we investigated the pro-oxidant effects of violacein in non-tumor (CHO-K1 and MRC-5) and tumor (HeLa) cell lines, searching for a better understanding of reactive oxygen species (ROS) production and cell death induction. Results Cytotoxicity induced by violacein was observed in the three cell lines; however, MRC-5 and HeLa cells were shown to be more sensitive to violacein treatment. Although punctual alterations in the antioxidant apparatus and increase in oxidative stress biomarkers was observed in some violacein concentrations, no association was found between increased oxidative stress and induction of cell death. However, the increase of mitochondrial membrane potential was observed. Conclusions In fact, the increase of mitochondrial membrane potential in MRC-5 and HeLa cells suggests that mitochondrial membrane hyperpolarization might be the main cause of cell death triggered by violacein. |
| Databáze: | OpenAIRE |
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