Talin tension sensor reveals novel features of focal adhesion force transmission and mechanosensitivity
| Autor: | Abhishek Kumar, Koen van den Dries, Benjamin T. Goult, Alex Groisman, Keiichiro Tanaka, Martin A. Schwartz, Mingxing Ouyang, Ewan J. McGhee, Marie D. Anderson, Kurt I. Anderson |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Talin animal structures Mechanotransduction Integrin macromolecular substances Medical and Health Sciences Mechanotransduction Cellular Models Biological Focal adhesion 03 medical and health sciences Mice 0302 clinical medicine Models Fluorescence Resonance Energy Transfer Animals Actin QH581.2 Focal Adhesions Binding Sites biology Tension (physics) Correction Cell Biology Biological Sciences Vinculin Biological Cell biology Actin Cytoskeleton 030104 developmental biology embryonic structures biology.protein NIH 3T3 Cells Mechanosensitive channels Cellular Nanomedicine Radboud Institute for Molecular Life Sciences [Radboudumc 19] 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | The Journal of Cell Biology Schwartz, M 2016, ' Talin tension sensor reveals novel features of focal adhesion force transmission and mechanosensitivity ', The Journal of cell biology, vol. 213, no. 3, PMID 27161398, pp. 371-83 . https://doi.org/10.1083/jcb.201510012 The Journal of cell biology, vol 213, iss 3 Journal of Cell Biology, 213, 371-83 Journal of Cell Biology, 213, 3, pp. 371-83 |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Contains fulltext : 171617.pdf (Publisher’s version ) (Open Access) Integrin-dependent adhesions are mechanosensitive structures in which talin mediates a linkage to actin filaments either directly or indirectly by recruiting vinculin. Here, we report the development and validation of a talin tension sensor. We find that talin in focal adhesions is under tension, which is higher in peripheral than central adhesions. Tension on talin is increased by vinculin and depends mainly on actin-binding site 2 (ABS2) within the middle of the rod domain, rather than ABS3 at the far C terminus. Unlike vinculin, talin is under lower tension on soft substrates. The difference between central and peripheral adhesions requires ABS3 but not vinculin or ABS2. However, differential stiffness sensing by talin requires ABS2 but not vinculin or ABS3. These results indicate that central versus peripheral adhesions must be organized and regulated differently, and that ABS2 and ABS3 have distinct functions in spatial variations and stiffness sensing. Overall, these results shed new light on talin function and constrain models for cellular mechanosensing. |
| Databáze: | OpenAIRE |
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