Single-Cell Microarray Analysis in Hippocampus CA1: Demonstration and Validation of Cellular Heterogeneity
| Autor: | Anton Bittner, Jessica Zhu, Mark G. Erlander, Lin Luo, Da-Thao Tran, Hongqing Guo, Fredrik Kamme, Ranelle C. Salunga, Jackson S. Wan, Nancy E. Miller, Jingxue Yu |
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| Rok vydání: | 2003 |
| Předmět: |
Cell type
Population Cell Count Computational biology Biology Hippocampus Sensitivity and Specificity Interneurons Bacteriophage T7 Complementary DNA Animals Cluster Analysis RNA Antisense RNA Messenger ARTICLE education Gene In Situ Hybridization Oligonucleotide Array Sequence Analysis Laser capture microdissection Neurons education.field_of_study Reverse Transcriptase Polymerase Chain Reaction Microarray analysis techniques Lasers Pyramidal Cells General Neuroscience Reproducibility of Results Molecular biology Rats Gene expression profiling nervous system Female DNA microarray |
| Zdroj: | The Journal of Neuroscience. 23:3607-3615 |
| ISSN: | 1529-2401 0270-6474 |
| Popis: | Laser capture microdissection in combination with microarrays allows for the expression analysis of thousands of genes in selected cells. Here we describe single-cell gene expression profiling of CA1 neurons in the rat hippocampus using a combination of laser capture, T7 RNA amplification, and cDNA microarray analysis. Subsequent cluster analysis of the microarray data identified two different cell types: pyramidal neurons and an interneuron. Cluster analysis also revealed differences among the pyramidal neurons, indicating that even a single cell type in vivo is not a homogeneous population of cells at the gene expression level. Microarray data were confirmed by quantitative RT-PCR and in situ hybridization. We also report on the reproducibility and sensitivity of this combination of methods. Single-cell gene expression profiling offers a powerful tool to tackle the complexity of the mammalian brain. |
| Databáze: | OpenAIRE |
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