The Relationship between Homology Length and Crossing Over during the Repair of a Broken Chromosome
| Autor: | Martin Kupiec, Ori Inbar, Gili Bitan, Batia Liefshitz |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Time Factors Unequal crossing over DNA Repair Base Pair Mismatch DNA repair Gene Conversion Saccharomyces cerevisiae Biology Biochemistry Chromosomes Chromosomal crossover Fungal Proteins Homology directed repair Crossing Over Genetic Gene conversion Molecular Biology Alleles Recombination Genetic Genetics Models Genetic DNA Restriction Enzymes Cell Biology DNA-Binding Proteins Blotting Southern Kinetics MutS Homolog 2 Protein MSH2 Mutation Mutagenesis Site-Directed DNA mismatch repair Homologous recombination Plasmids |
| Zdroj: | Journal of Biological Chemistry. 275:30833-30838 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.c000133200 |
| Popis: | Homologous recombination can result in the transfer of genetic information from one DNA molecule to another (gene conversion). These events are often accompanied by a reciprocal exchange between the interacting molecules (termed "crossing over"). This association suggests that the two types of events could be mechanistically related. We have analyzed the repair, by homologous recombination, of a broken chromosome in yeast. We show that gene conversion can be uncoupled from crossing over when the length of homology of the interacting substrates is below a certain threshold. In addition, a minimal length of homology on each broken chromosomal arm is needed for crossing over. We also show that the coupling between gene conversion and crossing over is affected by the mismatch repair system; mutations in the MSH2 or MSH6 genes cause an increase in the crossing over observed for short alleles. Our results provide a mechanism to explain how chromosomal recombinational repair can take place without altering the stability of the genome. |
| Databáze: | OpenAIRE |
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