| Autor: |
Francisco Campos‐Maldonado, María L. González‐Dávalos, Enrique Piña, Miriam Aracely Anaya‐Loyola, Armando Shimada, Alfredo Varela‐Echavarria, Ofelia Mora |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Journal of food biochemistryREFERENCES. |
| ISSN: |
1745-4514 |
| Popis: |
The goal of this study was to evaluate how glucose and fructose affected the adipose differentiation of pig newborn mesenchymal stem cells (MSCs). Cells were grown with or without inosine in 7.5 mM glucose (substituted with 1.5 or 6 mM fructose). MSCs displayed adipose morphology after 70 days of differentiation. Fructose stimulated the highest levels of PPARγ and C/EBPβ. Fructose at 6 mM, but not glucose at 7.5 mM or fructose at 1.5 mM, promotes differentiation of MSCs into adipocytes and increases 11-hydroxysteroid dehydrogenase (11β-HSD1) and NADPH oxidase 4 (NOX4) mRNA in the absence of hepatic effects (as simulated by the inosine). Fructose and glucose increased xanthine oxide-reductase (XOR) catalytic activity almost 10-fold and elevated their products: intracellular reactive oxygen species (ROS) pool, extracellular H |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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