A non-radiolabelled in situ hybridization method for the detection of epidermal cytokine mRNA
| Autor: | Sarah E. M. Howie, Eva McVittie, E. Ramage, E. Thornton, John A. A. Hunter, R. D. Aldridge |
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| Rok vydání: | 1992 |
| Předmět: |
In situ
Adult Male Biopsy Dermatology In situ hybridization Biology Dermatitis Contact Biochemistry chemistry.chemical_compound Exon Nickel Paraffin section Humans RNA Messenger Fluorescein Molecular Biology In Situ Hybridization Skin integumentary system Oligonucleotide Middle Aged Contact sensitivity Molecular biology chemistry Cytokines Cytokine mrna Female Epidermis Oligonucleotide Probes Fluorescein-5-isothiocyanate |
| Zdroj: | Experimental dermatology. 1(5) |
| ISSN: | 0906-6705 |
| Popis: | We describe a non-radiolabelled method for the in situ detection of epidermal cytokine mRNA in paraffin sections of skin biopsies from sites of nickel contact sensitivity. The method uses fluorescein isothiocyan-ate-labelled oligonucleotide exon probe cocktails. |
| Databáze: | OpenAIRE |
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