Nosema neumanni n. sp. (Microsporidia, Nosematidae), a new microsporidian parasite of honeybees, Apis mellifera in Uganda
Autor: | Lina De Smet, Moses Chemurot, Dirk C. de Graaf, Marleen Brunain, Riet De Rycke |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
EUROPE Nosematidae CERANAE MICROSPORIDIA Zoology Ultra-structure 16 SSU RNA SEQUENCE Microbiology 03 medical and health sciences Microscopy Electron Transmission Nosema Species Specificity RNA Ribosomal 16S Nosemosis INFECTION parasitic diseases Botany Animals Parasite hosting Uganda BEE COLONIES biology fungi Nosema apis Biology and Life Sciences Bees biology.organism_classification Nosema ceranae Microsporidium 030104 developmental biology African honeybee Microsporidia Polar filament |
Zdroj: | EUROPEAN JOURNAL OF PROTISTOLOGY |
ISSN: | 0932-4739 1618-0429 |
DOI: | 10.1016/j.ejop.2017.07.002 |
Popis: | The microsporidium Nosema neumanni n. sp., a new parasite of the honeybee Apis mellifera is described based on its ultra structural and molecular characteristics. Structures resembling microsporidian spores were found by microscopic examination of honeybees from Uganda. Molecular confirmation failed when PCR primers specific for Nosema apis and Nosema ceranae were used, but was successful with primers covering the whole family of Nosematidae. We performed transmission electron microscopy and found typical microsporidian spores which were smaller (length: 2.36 +/- 0.14 m and width: 1.78 +/- 0.06 p.m; n = 6) and had fewer polar filament coils (10-12) when compared to those of known species infecting honeybees. The entire 16S SS(J rRNA region was amplified, cloned and sequenced and was found to be unique with the highest resemblance (97% identity) to N. apis, The incidence of N. neumanni n. sp. in Ugandan honeybees was found to be much higher than of the two other Nosema species. |
Databáze: | OpenAIRE |
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