Is ribosome synthesis controlled by pol I transcription?
| Autor: | Arnaud Laferté, Christophe Carles, Tran Hoang, Denis L. J. Lafontaine, Stéphane Chédin, Michel Riva |
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| Přispěvatelé: | Laboratoire Régulation de l'Expression des Gènes et Epigénétique (LREGE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de Biologie et de Médecine Moléculaires, Université libre de Bruxelles (ULB) |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Transcription
Genetic MESH: RNA Polymerase I Ribosome biogenesis RNA polymerase II Biology Ribosome RNA polymerase III 03 medical and health sciences 0302 clinical medicine Ribosomal protein RNA Polymerase I RNA polymerase I Transcriptional regulation Animals Humans MESH: Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Molecular Biology 030304 developmental biology Genetics 0303 health sciences MESH: Humans MESH: Transcription Genetic Cell Biology Processivity 030220 oncology & carcinogenesis biology.protein Ribosomes MESH: Ribosomes Developmental Biology |
| Zdroj: | Cell Cycle Cell Cycle, 2007, 6 (1), pp.11-5 Cell Cycle, Taylor & Francis, 2007, 6 (1), pp.11-5 |
| ISSN: | 1538-4101 1551-4005 |
| Popis: | Regulation of growth ultimately depends on the control of synthesis of new ribosomes. Ribosome biogenesis is thus a key element of cell biology, which is tightly regulated in response to environmental conditions. In eukaryotic cells, the supply of ribosomal components involves the activities of the three forms of nuclear RNA polymerase (Pol I, Pol II and Pol III). Recently, we demonstrated that upon rapamycin treatment, a partial derepression of Pol I transcription led to a concomitant derepression of Pol II transcription restricted to a small subset of class II genes encompassing the genes encoding all ribosomal proteins, and 19 additional genes. The products of 14 of these 19 genes are principally involved in rDNA structure, ribosome biogenesis or translation, whereas the five remaining genes code for hypothetical proteins. We demonstrate that the proteins encoded by these five genes are required for optimal pre-rRNA processing. In addition, we show that cells in which regulation of Pol I transcription was specifically impaired are either resistant or hypersensitive to different stresses compared to wild-type cells. These results highlight the critical role of the regulation of Pol I activity for the physiology of the cells. |
| Databáze: | OpenAIRE |
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