Diversity and antibiotic resistance patterns of Sphingomonadaceae isolates from drinking water
| Autor: | Olga C. Nunes, Célia M. Manaia, Ivone Vaz-Moreira |
|---|---|
| Přispěvatelé: | Faculdade de Engenharia, Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Disease reservoir
Microbiologia Engenharia química Ciências biológicas Novosphingobium Blastomonas Molecular Sequence Data Public Health Microbiology 010501 environmental sciences 01 natural sciences Applied Microbiology and Biotechnology Dental Equipment Sphingobium Microbiology 03 medical and health sciences Antibiotic resistance Microbiology Chemical engineering Biological sciences Drug Resistance Multiple Bacterial RNA Ribosomal 16S Cluster Analysis Biological sciences [Natural sciences] 0105 earth and related environmental sciences Disease Reservoirs 0303 health sciences Ecology biology Base Sequence 030306 microbiology Drinking Water Genetic Variation Genes rRNA biology.organism_classification Sphingomonas 6. Clean water Bacterial Typing Techniques Culture Media Sphingomonadaceae Vertical resistance Phenotype Genes Bacterial Biofilms Ciências biológicas [Ciências exactas e naturais] Water Microbiology Food Science Biotechnology |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | Sphingomonadaceae ( n = 86) were isolated from a drinking water treatment plant ( n = 6), tap water ( n = 55), cup fillers for dental chairs ( n = 21), and a water demineralization filter ( n = 4). The bacterial isolates were identified based on analysis of the 16S rRNA gene sequence, and intraspecies variation was assessed on the basis of atpD gene sequence analysis. The isolates were identified as members of the genera Sphingomonas ( n = 27), Sphingobium ( n = 28), Novosphingobium ( n = 12), Sphingopyxis ( n = 7), and Blastomonas ( n = 12). The patterns of susceptibility to five classes of antibiotics were analyzed and compared for the different sites of isolation and taxonomic groups. Colistin resistance was observed to be intrinsic (92%). The highest antibiotic resistance prevalence values were observed in members of the genera Sphingomonas and Sphingobium and for beta-lactams, ciprofloxacin, and cotrimoxazole. In tap water and in water from dental chairs, antibiotic resistance was more prevalent than in the other samples, mainly due to the predominance of isolates of the genera Sphingomonas and Sphingobium . These two genera presented distinct patterns of association with antibiotic resistance, suggesting different paths of resistance development. Antibiotic resistance patterns were often related to the species rather than to the site or strain, suggesting the importance of vertical resistance transmission in these bacteria. This is the first study demonstrating that members of the family Sphingomonadaceae are potential reservoirs of antibiotic resistance in drinking water. |
| Databáze: | OpenAIRE |
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