Additional file 1: of Human platelet lysate in mesenchymal stromal cell expansion according to a GMP grade protocol: a cell factory experience

Autor: Becherucci, Valentina, Piccini, Luisa, Casamassima, Serena, Bisin, Silvia, Gori, Valentina, Gentile, Francesca, Ceccantini, Riccardo, Rienzo, Elena De, Bindi, Barbara, Pavan, Paola, Cunial, Vanessa, Allegro, Elisa, Ermini, Stefano, Brugnolo, Francesca, Astori, Giuseppe, Bambi, Franco
Rok vydání: 2018
Předmět:
DOI: 10.6084/m9.figshare.6215423.v1
Popis: Figure S1. Evaluation of Treg cell induction by MSCs expanded in PL with two different methods. Data presented as mean ± SD with n = 6. Treg induction evaluated after 7 days of coculture with MSC as % CD4+/CD25High/CD127Low PBMCs versus Í4+/CD25High/Foxp3+ PBMCs. No significant differences (p > 0.05) found between the two methods concerning identification of Treg cells. Foxp3 staining of PBMCs performed with the BD Pharmingen™ Anti-Human FoxP3 Staining Kit. MSCs cultured with PL were able to induce the Treg cell population if compared with PBMCs alone (*p
Databáze: OpenAIRE