Effects of endogenous and exogenous D1/D5 dopamine receptor activation on LTP in ventral and dorsal CA1 hippocampal synapses
| Autor: | Costas Papatheodoropoulos, Vassilios Papaleonidopoulos, Stylianos Kouvaros |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Agonist Male medicine.drug_class Dopamine Agents Long-Term Potentiation Tissue Culture Techniques 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine Dopamine receptor D1 Dopamine medicine Animals Receptors Dopamine D5 RNA Messenger Rats Wistar SCH-23390 Dose-Response Relationship Drug Chemistry Dopaminergic Excitatory Postsynaptic Potentials Long-term potentiation Cell biology 030104 developmental biology nervous system Dopamine receptor Synaptic plasticity Synapses 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Synapse (New York, N.Y.). 72(8) |
| ISSN: | 1098-2396 |
| Popis: | Hippocampus is importantly involved in dopamine-dependent behaviors and dopamine is a significant modulator of synaptic plasticity in the hippocampus. Moreover, the dopaminergic innervation appears to be disproportionally segregated along the hippocampal longitudinal (dorsoventral) axis with unknown consequences for synaptic plasticity. In this study we examined the actions of endogenously released dopamine and the effects of exogenous D1/D5 dopamine receptor agonists on theta-burst stimulation-induced long-term potentiation (LTP) of field excitatory synaptic potential (fEPSP) at Schaffer collateral-CA1 synapses in slices from dorsal (DH) and ventral hippocampus (VH). Furthermore, we quantified D1 receptor mRNA and protein expression levels in DH and VH. We found that blockade of D1/D5 receptors by SCH 23390 (20 μM) significantly reduced the magnitude of LTP in both DH and VH similarly suggesting that dopamine endogenously released during TBS, presumably mimicking low activity of DA neurons, exerts a homogeneous modulation of LTP along the hippocampal long axis. Moderate to high concentrations of the selective partial D1/D5 receptor agonist SKF 38393 (50-150 μM) did not significantly change LTP in either hippocampal segment. However, the full D1 receptor selective agonist SKF 82958 (10 μM) significantly enhanced LTP in VH but not DH. Furthermore, the expression of D1 receptor mRNA and protein was considerably higher in VH compared with DH. These results suggest that the dynamic range of D1/D5 receptor-mediated dopamine effects on LTP may be higher in VH than DH and that VH may be specialized to acquire information about behaviorally relevant strong stimuli signaled by the dopamine system. |
| Databáze: | OpenAIRE |
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