Correlation between store-operated cation current and capacitative Ca2+ influx in smooth muscle cells from mouse anococcygeus
| Autor: | Alan Gibson, Ian McFadzean, Patrick Wallace, Christopher P. Wayman |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Indoles Patch-Clamp Techniques Carbachol Vasodilator Agents chemistry.chemical_element Ion Pumps Calcium Second Messenger Systems Tonic (physiology) Mice chemistry.chemical_compound Internal medicine medicine Animals Pharmacology Chemistry Imidazoles Muscle Smooth Microfluorimetry Calcium Channel Blockers Electrophysiology Endocrinology Biophysics medicine.symptom Cyclopiazonic acid Intracellular Muscle contraction medicine.drug |
| Zdroj: | European Journal of Pharmacology. 376:325-329 |
| ISSN: | 0014-2999 |
| Popis: | In mouse anococcygeus cells, simultaneous measurements of membrane currents and changes in intracellular Ca 2+ were obtained using “perforated-patch” whole-cell recordings and Fura-2 microfluorimetry. Carbachol (50 μM) and cyclopiazonic acid (10 μM) produced a biphasic inward current; a transient Ca 2+ -dependent chloride current ( I ClCa ), followed by a smaller, sustained current ( I DOC ). This response was mirrored by a biphasic increase in the intracellular Ca 2+ concentration. SKF96365 (1-{β-[3-(4-methoxyphenyl) propoxyl]-4-methoxyphenethyl}-1 H -imidazole; 10 μM) and Cd 2+ (100 μM) inhibited both I DOC and the sustained increase in intracellular Ca 2+ ; La 3+ (400 μM) inhibited neither response. The results confirm that the non-selective cation current I DOC underlies capacitative Ca 2+ influx supporting sustained contractions in this tonic smooth muscle. |
| Databáze: | OpenAIRE |
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