Can rapid dengue diagnostic kits be trusted? A comparative study of commercially available rapid kits for serodiagnosis of dengue fever
| Autor: | T.N. Dhole, Dharam Veer Singh, Atul Garg, Jaya Garg |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Igm antibody
business.industry lcsh:R Ns1 antigen Febrile illness lcsh:Medicine Diagnostic accuracy NS1 antigen Dengue virus medicine.disease_cause Serum samples medicine.disease Virology Serology Dengue fever Dengue rapid diagnostic test kits medicine Original Article IgM antibodies enzyme-linked immunosorbent assay business |
| Zdroj: | Journal of Laboratory Physicians Journal of Laboratory Physicians, Vol 11, Iss 01, Pp 063-067 (2019) |
| ISSN: | 0974-2727 |
| Popis: | BACKGROUND: Dengue virus infection is an important emerging disease of the tropical and subtropical regions and is mainly diagnosed by serological detection of NS1 antigen and IgM antidengue antibodies. Since enzyme-linked immunosorbent assay (ELISA) facilities are not easily available at most diagnostic centers, so most of them use various commercially available rapid diagnostic tests (RDTs) kits. AIMS AND OBJECTIVES: This study was designed to access the diagnostic accuracy of four commercially available and widely used RDTs for serodiagnosis of dengue virus infection in Indian laboratories. SUBJECTS AND METHODS: The study was conducted at Department of Microbiology, G.S.V.M Medical College, Kanpur, India, to estimate the sensitivity and specificity of following RDTs: (1) Dengue Cassette (Panbio, Australia), (2) Bioline Dengue Duo (SD Diagnostics, Korea), (3) Dengue Day 1 test (J Mitra and Co., India), and (4) Dengucheck Duo (Tulip Diagnostics, India) on 72 confirmed dengue serum samples that were positive by dengue reverse transcription-polymerase chain reaction, dengue NS1, and IgM ELISA along with 80 serum samples from nondengue febrile illness patients. RESULTS: The majority of the RDTs demonstrated low sensitivity but good specificity for detecting NS1 antigen. Detection of antidengue IgM antibodies by RDTs demonstrated low sensitivity ranging from 27.8% to 77.7%. However, specificity was generally higher (50%–86.2%) and more consistent across the assays. CONCLUSION: The study results differed markedly from the RDTs manufacturers’ claimed performance characteristics. Therefore, the RDT results should be interpreted cautiously and ELISA should be performed as far as possible for serodiagnosis of dengue virus infection. |
| Databáze: | OpenAIRE |
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