Online monitoring of metabolism and morphology of peptide-treated neuroblastoma cancer cells and keratinocytes
| Autor: | Sabine Drechsler, Jörg Andrä |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Keratinocytes
Programmed cell death Physiology Antimicrobial peptides Cell Peptide Biology Melittin Cell Line chemistry.chemical_compound Neuroblastoma Cell Line Tumor medicine Humans chemistry.chemical_classification Cell Biology medicine.disease Melitten Cell biology HaCaT medicine.anatomical_structure chemistry Cancer cell Peptides Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Journal of bioenergetics and biomembranes. 43(3) |
| ISSN: | 1573-6881 |
| Popis: | Antimicrobial peptides are promising anti-cancer agents with a unique mode of action. We established the usage of a chip-based sensor to monitor the dynamic interplay between cells on the chip and peptides and compared it with endpoint tests. Human neuroblastoma cancer cells and spontaneously immortalized non-cancer keratinocytes were perfused with representative peptides (NK-2, NK11, and melittin). The sensor system enabled continuous recording of cell layer impedance (adhesion/confluence), oxygen consumption (respiration) and extracellular acidification (glycolysis) and provided insights in cell damage, stress response and recovery. Cells responded differentially to peptide treatment. During perfusion, peptides accumulated on the cell surface until they reached a critical concentration. Preceding to cell death, melittin triggered glycolysis, suggesting stress response. NK-2 induced no change in energy metabolism, but led to an increase in impedance, i.e. a temporarily altered morphology, which appeared to be an excellent parameter to detect subtle structural changes of cell layers. |
| Databáze: | OpenAIRE |
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