ANTIGENIC SIMILARITIES BOTH INSIDE AND OUTSIDE THE CARBOHYDRATE-BINDING SITES OF TWO-CHAIN AND ONE-CHAIN LEGUMINOUS LECTINS
| Autor: | Jan Kolberg, Terje E. Michaelsen |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Immunodiffusion
Chemical Phenomena Hemagglutination Immunology Enzyme-Linked Immunosorbent Assay Biology Lymphocyte Activation Antibodies Peanut Agglutinin chemistry.chemical_compound Antigen Lectins Concanavalin A Immune Tolerance Animals Humans Antigens Phytohemagglutinins Binding site Guanidine Binding Sites Plants Medicinal General Immunology and Microbiology Lectin Fabaceae General Medicine Hemagglutination Inhibition Tests Carbohydrate Molecular biology Chemistry chemistry Biochemistry Galactose biology.protein Carbohydrate Metabolism Rabbits Plant Lectins Antibody |
| Zdroj: | Acta Pathologica Microbiologica Scandinavica Series C: Immunology. :25-35 |
| ISSN: | 0108-0202 |
| DOI: | 10.1111/j.1699-0463.1984.tb00048.x |
| Popis: | Antibodies were made against the two-chain lectin Lath-O from the seeds of Lathyrus odoratus as well as its isolated light (alpha) and heavy (beta) chains. These antibodies were used to antigenically compare the Lath-O with other two-chain lectins like Lath-S, lentil and Vicia cracca glc specific and with one-chain lectins like Con A, PHA, peanut and soybean. Sensitive ELISA tests showed that there was a distinct antigenic cross-reaction between the one-chain and two-chain lectins both by using antibodies against Lath-O whole molecules and its isolated beta-chains, while the anti-Lath-O alpha-chain antibodies barely reacted at all even with Lath-O alpha-chains. The antibodies against Lath-O whole molecules also strongly inhibited the mitogenic responses induced by two-chain lectins. Antibodies reacting inside the carbohydrate-binding site of the lectins were isolated by eluting them with glucose from lectin-Sepharose columns and antibodies reacting outside the carbohydrate-binding site were subsequently eluted with guanidine or buffer with low pH. The two antibody fractions were separately used to antigenically compare one-chain and two-chain lectins, demonstrating that both kinds of antibodies showed cross-reaction between one-chain and two-chain lectins. Both the antibodies presumably reacting inside the carbohydrate-binding site of the lectins and those reacting outside the carbohydrate-binding site inhibited haemagglutination activity of two-chain lectins, while haemagglutination by one-chain lectins was unaffected. The carbohydrate-binding site specificity of the glucose-eluted antibodies was assessed by glucose-inhibition in ELISA tests. Furthermore, galactose neither inhibited in these tests nor eluted antibodies from lectin-Sepharose columns. Control experiments were also performed with normal rabbit IgG. |
| Databáze: | OpenAIRE |
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