Evidence of Alternative Splicing as a Regulatory Mechanism for Kissr2 in Pejerrey Fish
| Autor: | Guillermo Ortí, Eda Suku, María Rita Pérez, Ariel E. Mechaly, M. Oswaldo Tovar Bohórquez, Gustavo M. Somoza, Jordi Viñas, Alejandro Giorgetti, Alejandro S. Mechaly |
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| Přispěvatelé: | Instituto de Investigaciones Biotecnológicas [San Martín] (IIB-INTECH), Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET)-Universidad Nacional de San Martin (UNSAM), Cristallographie (Plateforme) - Crystallography (Platform), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), University of Verona (UNIVR), The George Washington University (GW), Universitat de Girona (UdG), This study was funded by projects grants by the Agencia Nacional de Promoción Científica y Tecnológica (ANPCYT, Argentina) to ASM (PICT-2014-0515) and to GMS (PICT-2012-2619 and PICT-2015-2783)., Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Università degli studi di Verona = University of Verona (UNIVR) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
fasting Endocrinology Diabetes and Metabolism Biology lcsh:Diseases of the endocrine glands. Clinical endocrinology purl.org/becyt/ford/1 [https] Ciencias Biológicas 03 medical and health sciences Exon alternative splicing GPCR Kisspeptin Endocrinology MODELING Gene expression KISSR3 FASTING [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology KISSR2 ALTERNATIVE SPLICING kisspeptin receptors purl.org/becyt/ford/1.6 [https] Gene Original Research Genetics lcsh:RC648-665 GOMODO kissr3 Alternative splicing Intron modeling Bioquímica y Biología Molecular kissr2 Bioinformatics modeling KISSPEPTIN RECEPTORS Stop codon 030104 developmental biology RNA splicing Isoforms PEJERREY FISH CIENCIAS NATURALES Y EXACTAS pejerrey fish GoMoDo |
| Zdroj: | Frontiers in Endocrinology Frontiers in Endocrinology, Frontiers, 2018, 9, pp.604. ⟨10.3389/fendo.2018.00604⟩ Frontiers in Endocrinology, Vol 9 (2018) CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Frontiers in Endocrinology, 2018, 9, pp.604. ⟨10.3389/fendo.2018.00604⟩ |
| ISSN: | 1664-2392 |
| DOI: | 10.3389/fendo.2018.00604⟩ |
| Popis: | Kisspeptin receptors are G-Protein-Coupled Receptors that regulate GnRH synthesis and release in vertebrates. Here, we report the gene structure of two kisspeptin receptors (kissr2 and kissr3) in pejerrey fish. Genomic analysis exposed a gene structure with 5 exons and 4 introns for kissr2 and 6 exons and 5 introns for kissr3. Two alternative variants for both genes, named kissr2_v1 and _v2, and kissr3_v1 and v2, were revealed by gene expression analyses of several tissues. For both receptors, these variants were originated by alternative splicing retaining intron 3 and intron 4 for kissr2_v2 and kissr3_v2, respectively. In the case of kissr2, the intron retention introduced two stop codons leading to a putatively truncated protein whereas for kissr3, the intron retention produced a reading shift leading to a stop codon in exon 5. Modeling and structural analysis of Kissr2 and Kissr3 spliced variants revealed that truncation of the proteins may lead to non-functional proteins, as the structural elements missing are critical for receptor function. To understand the functional significance of splicing variants, the expression pattern for kissr2 was characterized on fish subjected to different diets. Fasting induced an up-regulation of kissr2_v1 in the hypothalamus, a brain region implicated in control of reproduction and food intake, with no expression of kissr2_v2. On the other hand, fasting did not elicit differential expression in testes and habenula. These results suggest that alternative splicing may play a role in regulating Kissr2 function in pejerrey. Fil: Mechaly, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina Fil: Bohórquez, M. Oswaldo Tovar. Instituto Nacional de Investigaciones y Desarrollo Pesquero; Argentina Fil: Mechaly, Ariel Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Suku, Eda. Centre National de la Recherche Scientifique; Francia Fil: Pérez, María Rita. Universita di Verona; Italia Fil: Giorgetti, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina Fil: Orti, Guillermo. Universita di Verona; Italia Fil: Viñas de Puig, Jordi. Universidad de Girona; España Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina |
| Databáze: | OpenAIRE |
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