Sulfated polysaccharide from the green marine algae Caulerpa racemosa reduces experimental pain in the rat temporomandibular joint
| Autor: | Felipe Dantas Silveira, Lorena Vasconcelos Vieira, Norma Maria Barros Benevides, Hellíada Vasconcelos Chaves, Renata Line da Conceição Rivanor, Ellen Lima de Assis, Natássia Albuquerque Ribeiro, Gerly Anne de Castro Brito, Hermany Capistrano Freitas, Danielle Rocha do Val, Francisco Isaac Fernandes Gomes, Deiziane Viana da Silva Costa, Mirna Marques Bezerra |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Aquatic Organisms Vascular permeability Inflammation 02 engineering and technology Pharmacology Nitric Oxide Biochemistry Nitric oxide 03 medical and health sciences chemistry.chemical_compound Western blot Chlorophyta Polysaccharides Structural Biology medicine Animals Molecular Biology 030304 developmental biology Analgesics 0303 health sciences Temporomandibular Joint medicine.diagnostic_test Sulfates Chemistry General Medicine 021001 nanoscience & nanotechnology Arthralgia Extravasation Rats Nociception Capsaicin Cytokines Serotonin Inflammation Mediators medicine.symptom 0210 nano-technology Biomarkers Heme Oxygenase-1 |
| Zdroj: | International Journal of Biological Macromolecules. 150:253-260 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2020.01.272 |
| Popis: | Temporomandibular disorder is a clinical painful condition in the temporomandibular joint (TMJ) region. The purified sulfated polysaccharide from the green marine algae Caulerpa racemosa (Cr) has provided anti-inflammatory and antinociceptive activity. This study evaluated these effects on a TMJ hypernociception model. Wistar rats (180 - 250 g) were pre-treated (i.v.) with Cr at 0.01, 0.1, or 1 mg/kg or vehicle 30 min before formalin (1.5%/50 μL, i.art.), capsaicin (1.5%/20 μL, i.art.), or serotonin (225 μg/50 μL, i.art.) in the TMJ, and nociceptive behaviors were measured for 45 or 30 min upon inflammatory stimuli. Inflammatory parameters vascular permeability assay, TNF-α, and IL-1β by ELISA, protein expression of adhesion molecules ICAM-1 and CD55 by Western blot were assessed. The involvement of heme oxygenase-1 (HO-1) and nitric oxide (NO) pathways were assessed by pharmacological inhibition. Cr (1 mg/kg) reduced nociceptive behavior, plasmatic extravasation, TNF-α, and IL-1β levels, as well as ICAM-1 and CD55 expression in periarticular tissues. Cr antinociceptive effect was not prevented by aminoguanidine, but ZnPP-IX did reduce its antinociceptive effect. Therefore, Cr antinociceptive and anti-inflammatory effects in this experimental model of hypernociception depended on the HO-1 pathway integrity, as well as reducing peripheral inflammatory events, e.g., TNF-α and IL-1β cytokines levels, ICAM-1 and CD55 expression. |
| Databáze: | OpenAIRE |
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