Heparin/collagen surface coatings modulate the growth, secretome, and morphology of human mesenchymal stromal cell response to interferon-gamma
| Autor: | Priyanka Priyadarshani, Said J. Cifuentes, Jorge Almodovar, Luke J. Mortensen, Maribella Domenech, David A. Castilla-Casadiego |
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| Rok vydání: | 2020 |
| Předmět: |
Stromal cell
Materials science 0206 medical engineering Cell Biomedical Engineering Bone Marrow Cells 02 engineering and technology Biomaterials Interferon-gamma Coated Materials Biocompatible medicine Adipocytes Animals Humans Interferon gamma Secretion Cells Cultured Cell Proliferation Secretome Cell growth Heparin Mesenchymal stem cell Metals and Alloys Anticoagulants Mesenchymal Stem Cells biochemical phenomena metabolism and nutrition equipment and supplies 021001 nanoscience & nanotechnology 020601 biomedical engineering Molecular biology medicine.anatomical_structure Ceramics and Composites Cytokines Cattle Bone marrow Collagen 0210 nano-technology Intracellular Immunosuppressive Agents medicine.drug |
| Zdroj: | Journal of biomedical materials research. Part AREFERENCES. 109(6) |
| ISSN: | 1552-4965 |
| Popis: | The therapeutic potential of human mesenchymal stromal cells (h-MSC) is dependent on the viability and secretory capacity of cells both modulated by the culture environment. Our previous studies introduced heparin and collagen I (HEP/COL) alternating stacked layers as a potential substrate to enhance the secretion of immunosuppressive factors of h-MSCs. Herein, we examined the impact of HEP/COL multilayers on the growth, morphology, and secretome of bone marrow and adipose-derived h-MSCs. The physicochemical properties and stability of the HEP/COL coatings were confirmed at 0 and 30 days. Cell growth was examined using cell culture media supplemented with 2 and 10% serum for 5 days. Results showed that HEP/COL multilayers supported h-MSC growth in 2% serum at levels equivalent to 10% serum. COL and HEP as single component coatings had limited impact on cell growth. Senescent studies performed over three sequential passages showed that HEP/COL multilayers did not impair the replicative capacity of h-MSCs. Examination of 27 cytokines showed significant enhancements in eight factors, including intracellular indoleamine 2, 3-dioxygenase, on HEP/COL multilayers when stimulated with interferon-gamma (IFN-γ). Image-based analysis of cell micrographs showed that serum influences h-MSC morphology; however, HEP-ended multilayers generated distinct morphological changes in response to IFN-γ, suggesting an optical detectable assessment of h-MSCs immunosuppressive potency. This study supports HEP/COL multilayers as a culture substrate for undifferentiated h-MSCs cultured in reduced serum conditions. |
| Databáze: | OpenAIRE |
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