An all-fiber-optic endoscopy platform for simultaneous OCT and fluorescence imaging
| Autor: | Xingde Li, Jiefeng Xi, Jessica Mavadia, Yongping Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Materials science Fluorophore genetic structures ocis:(170.6280) Spectroscopy fluorescence and luminescence 02 engineering and technology 01 natural sciences 010309 optics chemistry.chemical_compound Optics Optical coherence tomography Wavelength-division multiplexing 0103 physical sciences Endomicroscopy medicine Medical imaging ocis:(170.4500) Optical coherence tomography ocis:(170.2150) Endoscopic imaging medicine.diagnostic_test business.industry 021001 nanoscience & nanotechnology Cladding (fiber optics) Fluorescence Atomic and Molecular Physics and Optics eye diseases chemistry sense organs 0210 nano-technology business Endoscopes Catheters and Micro-Optics Biotechnology |
| Zdroj: | Biomedical Optics Express |
| ISSN: | 2156-7085 |
| Popis: | We present an all-fiber-optically based endoscope platform for simultaneous optical coherence tomography (OCT) and fluorescence imaging. This design entails the use of double-clad fiber (DCF) in the endoscope for delivery of OCT source and fluorescence excitation light while collecting the backscattered OCT signal through the single-mode core and fluorescence emission through the large inner cladding of the DCF. Circumferential beam scanning was performed by rotating a 45° reflector using a miniature DC motor at the distal end of the endoscope. Additionally, a custom DCF coupler and a wavelength division multiplexer (WDM) were utilized to seamlessly integrate both imaging modalities to achieve an entirely fiber-optically based dual-modality imaging system. We demonstrated simultaneous intraluminal 3D OCT and 2D (surface) fluorescence imaging in ex vivo rabbit esophagus using the dual-modal endomicroscopy system. Structural morphologies (provided by OCT) and fluorophore distribution (provided by the fluorescence module) could be clearly visualized, suggesting the potential of the dual-modality system for future in vivo and clinical applications. |
| Databáze: | OpenAIRE |
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