ApoA-I-binding protein (AI-BP) and its homologues hYjeF_N2 and hYjeF_N3 comprise the YjeF_N domain protein family in humans with a role in spermiogenesis and oogenesis

Autor: Wolfram Gronwald, I. Verdorfer, Alexander Sigruener, Hans Robert Kalbitzer, Gerd Schmitz, Christian Rudolph, B. Seifert, Olaf Ortmann, Stefan R. Bornstein, M. Bals-Pratsch, Arndt Hartmann, C. M. Luetjens, Evelyn Orsó
Rok vydání: 2007
Předmět:
Male
Spermiogenesis
Endocrinology
Diabetes and Metabolism
Clinical Biochemistry
Pregnancy Proteins
Biochemistry
Endocrinology
Oogenesis
Testis
Promoter Regions
Genetic
Aged
80 and over
Leydig cell
Reverse Transcriptase Polymerase Chain Reaction
General Medicine
Middle Aged
Sertoli cell
Immunohistochemistry
medicine.anatomical_structure
Child
Preschool
Female
DNA Probes
Lipoproteins
HDL
Leydig Cell Tumor
Adult
endocrine system
medicine.medical_specialty
DNA
Complementary
Protein family
Protein domain
Blotting
Western
Molecular Sequence Data
Racemases and Epimerases
Fibroma
Biology
Testicular Neoplasms
Internal medicine
medicine
Humans
Amino Acid Sequence
Spermatogenesis
Aged
urogenital system
Genome
Human
Biochemistry (medical)
Ovary
Apical membrane
Blotting
Northern
RNA
Carrier Proteins
Steroid hormone metabolism
Zdroj: Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme. 39(5)
ISSN: 0018-5043
Popis: The screening for additional human YjeF_N domain containing proteins beside the apolipoprotein A-I interacting protein (AI-BP), identified two other genes designated hYjeF_N2-15q23 (formerly human homologue of yeast edc3) and hYjeF_N3-19p13.11 comprising the human YjeF_N family. AI-BP is ubiquitously expressed, with a predominance of these tissues where the homologues were found to be restricted including brain, mammary gland, testes and ovaries. Immunohistochemistry of human testes and ovaries showed an expression of hYjeF_N3-19p13.11 only in Leydig cells and theca cells, respectively, indicating a role in steroid hormone metabolism. Interestingly, the protein was also strongly expressed in Leydig cell tumors and in thecofibromas. The identification of hYjeF_N2-15q23 in theca cells and granulosa cells in ovaries, in human spermatids of meiotic division part II and the apical membrane of Sertoli cells in testes suggest similar functions in oogenesis and sperm maturation which is strengthened by the identification of the spermatogenesis regulator HMGA1 as a conserved transcription factor. However, in contrast to AI-BP, both homologous proteins are unable to bind apoA-I. These results relate the human YjeF_N domain containing protein family to cholesterol processing and steroid hormone metabolism in spermiogenesis and oogenesis, and AI-BP may link this function to the HDL pathway.
Databáze: OpenAIRE
Externí odkaz: