Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Autor: Sil Park, Reuben Kim, No-Hee Park, Minju Song, Mo Kang, Sol Kim, Terresa Kim, Ki-Hyuk Shin
Rok vydání: 2017
Předmět:
0301 basic medicine
Secondary
General Chemical Engineering
Dental Pulp Capping
Dentistry
Regenerative pulp biology
Reparative dentin
Dentin
Secondary

Pulp capping
General Biochemistry
Genetics and Molecular Biology

Mouse model
Mice
03 medical and health sciences
0302 clinical medicine
stomatognathic system
Dentin
Psychology
Animals
Medicine
Issue 119
Dental Pulp Exposure
Dental/Oral and Craniofacial Disease
Orthodontics
Micro-computed tomography
Wound Healing
Enamel paint
General Immunology and Microbiology
business.industry
General Neuroscience
Mineral trioxide aggregates
030206 dentistry
stomatognathic diseases
030104 developmental biology
medicine.anatomical_structure
visual_art
visual_art.visual_art_medium
Pulp (tooth)
Cognitive Sciences
Biochemistry and Cell Biology
Pulp wound healing
business
Wound healing
Zdroj: Journal of visualized experiments : JoVE, vol 2017, iss 119
ISSN: 1940-087X
DOI: 10.3791/54973-v
Popis: Dental pulp is a vital organ of a tooth fully protected by enamel and dentin. When the pulp is exposed due to cariogenic or iatrogenic injuries, it is often capped with biocompatible materials in order to expedite pulpal wound healing. The ultimate goal is to regenerate reparative dentin, a physical barrier that functions as a "biological seal" and protects the underlying pulp tissue. Although this direct pulp-capping procedure has long been used in dentistry, the underlying molecular mechanism of pulpal wound healing and reparative dentin formation is still poorly understood. To induce reparative dentin, pulp capping has been performed experimentally in large animals, but less so in mice, presumably due to their small sizes and the ensuing technical difficulties. Here, we present a detailed, step-by-step method of performing a pulp-capping procedure in mice, including the preparation of a Class-I-like cavity, the placement of pulp-capping materials, and the restoration procedure using dental composite. Our pulp-capping mouse model will be instrumental in investigating the fundamental molecular mechanisms of pulpal wound healing in the context of reparative dentin in vivo by enabling the use of transgenic or knockout mice that are widely available in the research community.
Databáze: OpenAIRE