Early barriers to neonatal porcine islet engraftment in a dual transplant model
| Autor: | Tam How, Francis Leopardi, Robert Patrick Davis, J. Cano, Benjamin M. Martin, Bradley H. Collins, K.D. Williams, Gayathri R. Devi, Kannan P. Samy, Anna G. McDonald, Qimeng Gao, Alton B. Farris, E.K. Gall, C.R. Dove, M. Song, Allan D. Kirk |
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| Rok vydání: | 2018 |
| Předmět: |
Graft Rejection
0301 basic medicine Swine Xenotransplantation medicine.medical_treatment Transplantation Heterologous Islets of Langerhans Transplantation Apoptosis 030230 surgery Article Islets of Langerhans 03 medical and health sciences 0302 clinical medicine Immune system medicine Animals Immunology and Allergy Macrophage Pharmacology (medical) Inflammation Transplantation geography TUNEL assay geography.geographical_feature_category business.industry Macrophages Graft Survival Islet Macaca mulatta Disease Models Animal 030104 developmental biology Animals Newborn Terminal deoxynucleotidyl transferase Immunology business |
| Zdroj: | American Journal of Transplantation. 18:998-1006 |
| ISSN: | 1600-6135 |
| Popis: | Porcine islet xenografts have the potential to provide an inexhaustible source of islets for β cell replacement. Proof-of-concept has been established in nonhuman primates. However, significant barriers to xenoislet transplantation remain, including the poorly understood instant blood-mediated inflammatory reaction and a thorough understanding of early xeno-specific immune responses. A paucity of data exist comparing xeno-specific immune responses with alloislet (AI) responses in primates. We recently developed a dual islet transplant model, which enables direct histologic comparison of early engraftment immunobiology. In this study, we investigate early immune responses to neonatal porcine islet (NPI) xenografts compared with rhesus islet allografts at 1 hour, 24 hours, and 7 days. Within the first 24 hours after intraportal infusion, we identified greater apoptosis (caspase 3 activity and TUNEL [terminal deoxynucleotidyl transferase dUTP nick end labeling])-positive cells) of NPIs compared with AIs. Macrophage infiltration was significantly greater at 24 hours compared with 1 hour in both NPI (wild-type) and AIs. At 7 days, IgM and macrophages were highly specific for NPIs (α1,3-galactosyltransferase knockout) compared with AIs. These findings demonstrate an augmented macrophage and antibody response toward xenografts compared with allografts. These data may inform future immune or genetic manipulations required to improve xenoislet engraftment. |
| Databáze: | OpenAIRE |
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