Expression and evaluation of porcine circovirus type 2 capsid protein mediated by recombinant adeno-associated virus 8
| Autor: | Yuhe Yin, Bo Wang, Shun Jiang, Shuang Li, Yongbo Qiao, Jiaojiao Nie, Wei Kong, Yaming Shan, Yuhua Shi, Xiaohui Lan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Circovirus
040301 veterinary sciences animal diseases viruses Immunology Porcine circovirus type 2 Biology medicine.disease_cause Immunoglobulin G Virus law.invention 0403 veterinary science 03 medical and health sciences Mice Immune system law medicine Animals Humans Gene Adeno-associated virus 030304 developmental biology 0303 health sciences Immunity Cellular Mice Inbred BALB C General Veterinary Viral Vaccines 04 agricultural and veterinary sciences recombinant adeno-associated virus 8 Dependovirus biology.organism_classification Virology capsid protein Immunity Humoral Porcine circovirus HEK293 Cells Capsid Recombinant DNA biology.protein Original Article Capsid Proteins |
| Zdroj: | Journal of Veterinary Science |
| ISSN: | 1976-555X 1229-845X |
| Popis: | Background Porcine circovirus type 2 (PCV2) is an important infectious pathogen implicated in porcine circovirus-associated diseases (PCVAD), which has caused significant economic losses in the pig industry worldwide. Objectives A suitable viral vector-mediated gene transfer platform for the expression of the capsid protein (Cap) is an attractive strategy. Methods In the present study, a recombinant adeno-associated virus 8 (rAAV8) vector was constructed to encode Cap (Cap-rAAV) in vitro and in vivo after gene transfer. Results The obtained results showed that Cap could be expressed in HEK293T cells and BABL/c mice. The results of lymphocytes proliferative, as well as immunoglobulin G (IgG) 2a and interferon-γ showed strong cellular immune responses induced by Cap-rAAV. The enzyme-linked immunosorbent assay titers obtained and the IgG1 and interleukin-4 levels showed that humoral immune responses were also induced by Cap-rAAV. Altogether, these results demonstrated that the rAAV8 vaccine Cap-rAAV can induce strong cellular and humoral immune responses, indicating a potential rAAV8 vaccine against PCV2. Conclusions The injection of rAAV8 encoding PCV2 Cap genes into muscle tissue can ensure long-term, continuous, and systemic expression. |
| Databáze: | OpenAIRE |
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