Genomic Organization of the Mouse OSF-1 Gene
| Autor: | Sunao Takeshita, Masahiro Sato, Toshimi Ito, Egon Amann, Koh-Ichi Katoh |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
RNA Splicing
Molecular Sequence Data Restriction Mapping Pleiotrophin Polymerase Chain Reaction Mice Exon Complementary DNA Genetics Animals Coding region Genomic library Amino Acid Sequence Cloning Molecular Promoter Regions Genetic Molecular Biology Gene Genomic organization Midkine Genomic Library Base Sequence Sequence Homology Amino Acid biology DNA Exons Cell Biology General Medicine Molecular biology Introns Blotting Southern biology.protein Cytokines Mitogens Carrier Proteins |
| Zdroj: | DNA and Cell Biology. 11:735-743 |
| ISSN: | 1557-7430 1044-5498 |
| Popis: | The mouse OSF-1 protein (also known as pleiotrophin, HB-GAM, HBGF-8, or HBNF) gene was isolated from a mouse genomic library and sequenced. OSF-1 is a 15-kD secreted protein specifically expressed in bone and brain, and is believed to play a role in brain development and osteogenesis. The mouse OSF-1 gene consists of at least 5 exons and 4 introns and spans32 kb. Computer analysis of approximately 4 kb of 5'-flanking sequence of the OSF-1 gene revealed two candidate promoter regions. One candidate promoter contains a thyroid hormone/retinoic acid-responsive element and the other contains two glucocorticoid-responsive elements. DNA sequence analysis of novel OSF-1 cDNA clones indicates that two promoters can be utilized in MC3T3-E1 osteoblastic cells. The overall organization of the mouse OSF-1 gene is similar and the locations of the three exon-intron junctions within the coding region are identical to the mouse gene encoding the differentiation-related factor midkine (MK). Based on this similarity and on the high degree of nucleotide sequence homology (approximately 55%) of mouse OSF-1 and mouse MK, we conclude that OSF-1 and MK are generated from a common ancestral gene and are members of a family of structurally and probably functionally related proteins. |
| Databáze: | OpenAIRE |
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