Identification of MaTu-MX agent as a new strain of lymphocytic choriomeningitis virus (LCMV) and serological indication of horizontal spread of LCMV in human population
| Autor: | Zuzana Závadová, Jan Zavada, Stefan Kaluz, Eva Závodská, Milota Kaluzová, Antony C. Willis, Silvia Pastorekova, Fedor Čiampor, Jaromir Pastorek, Lucia Reiserová |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
DNA Complementary Adolescent viruses Population Molecular Sequence Data Breast Neoplasms CHO Cells Biology Lymphocytic Choriomeningitis Lymphocytic choriomeningitis Antibodies Viral Virus Mice Viral Proteins Dogs Complementary DNA Virology Cricetinae medicine Disease Transmission Infectious Tumor Cells Cultured Animals Humans Lymphocytic choriomeningitis virus Amino Acid Sequence education Aged education.field_of_study Chinese hamster ovary cell 3T3 Cells Middle Aged medicine.disease Coculture Techniques Nucleoprotein Nucleoproteins biology.protein Vero cell RNA Viral Antibody HeLa Cells |
| Zdroj: | Virology. 257(1) |
| ISSN: | 0042-6822 |
| Popis: | In this study we elucidated the molecular character of MaTu-MX, previously described as an unusual transmissible agent. Amino acid sequencing of peptides generated from a 58-kDa MX-related protein purified from MaTu human carcinoma cells allowed us to identify it as a nucleoprotein (NP) of lymphocytic choriomeningitis virus (LCMV). Northern blot analysis detected LCMV-specific RNAs in MaTu cells. Comparative immunoprecipitations showed cross-reactivity between NP of LCMV strain WE and MX NP. Using RT-PCR, we have cloned MX NP cDNA. According to sequence comparison, MX LCMV is as closely related to both LCMV strains WE and Armstrong as these strains are to one another. Based on this finding we propose that MX is a new strain of LCMV. We also showed that the stability of MX NP in MaTu cells is very high and that the virus is transmissible by cell-to-cell contact or by cell-free extract to human HeLa and monkey Vero cells, but not to human AGS, canine MDCK, mouse NIH 3T3, and hamster CHO cells. Finally, employing MX LCMV NP in immunoprecipitation and solid-phase radioimmunoassay, we found 37.5% prevalence of anti-LCMV antibodies in human sera, suggesting possible horizontal spread of the virus in the human population. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect