A rat model of oral submucous fibrosis induced by bleomycin
| Autor: | Zhaojian Gong, Wei Xiong, Xiao Wang, Qian Min, Tian-You Ling, Shanshan Zhang, Chong-dai Luo |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.medical_treatment Blotting Western Rat model Oral Submucous Fibrosis Bleomycin Pathology and Forensic Medicine Immunoenzyme Techniques Rats Sprague-Dawley Transforming Growth Factor beta1 Interferon-gamma 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Microscopy Electron Transmission medicine Animals Radiology Nuclear Medicine and imaging Dentistry (miscellaneous) Interferon gamma 030212 general & internal medicine Saline business.industry medicine.disease Rats Blot Disease Models Animal chemistry Oral submucous fibrosis 030220 oncology & carcinogenesis Ultrastructure Female Surgery Collagen Oral Surgery business Myofibroblast Biomarkers medicine.drug |
| Zdroj: | Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology. 122:216-223 |
| ISSN: | 2212-4403 |
| DOI: | 10.1016/j.oooo.2015.07.042 |
| Popis: | Objective The aim of this study was to induce a rat model of oral submucous fibrosis (OSF) using bleomycin. Study Design A randomized trial was performed. Bleomycin dilution or phosphate-buffered saline was injected daily into the buccal mucosa of the test and control rats, respectively, for 2, 4, 6, or 8 weeks. Changes in histopathologic features, myofibroblasts, ultrastructure, and the levels of collagen type I and III, transforming growth factor–beta 1, and interferon-γ were measured. Results Compared with controls, the buccal mucosa of the test rats appeared pale and stiff. There was collagen deposition in the laminae propria. Type I and type III collagen increased steadily, transforming growth factor–beta 1 increased and then decreased, and interferon-γ was gradually reduced. The ultrastructure was also similar to that of OSF. Conclusions Using bleomycin, we induced a rat model of OSF that is very similar to human OSF in clinical manifestations, pathology, and ultrastructural changes. |
| Databáze: | OpenAIRE |
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