Cosmeceutical properties of levan produced by Zymomonas mobilis
| Autor: | Chul-Ho Kim, Ki Ho Kim, Chang Sung Han, Young Heui Kim, Ki Soo Kim, Chan Bok Chung |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adult
Keratinocytes Aging Molecular Sequence Data Anti-Inflammatory Agents Pharmaceutical Science Cosmetics Dermatology Polysaccharide Zymomonas mobilis Artificial skin chemistry.chemical_compound Colloid and Surface Chemistry Microscopy Electron Transmission Drug Discovery Hyaluronic acid medicine Humans Viability assay Nuclear Magnetic Resonance Biomolecular Cell Proliferation Skin chemistry.chemical_classification Zymomonas Emollients integumentary system biology Polysaccharides Bacterial Fructose Fibroblasts biology.organism_classification Fructans Molecular Weight medicine.anatomical_structure Carbohydrate Sequence chemistry Biochemistry Chemistry (miscellaneous) Female Keratinocyte Cosmeceutical |
| Zdroj: | International Journal of Cosmetic Science. 28:231-231 |
| ISSN: | 1468-2494 0142-5463 |
| DOI: | 10.1111/j.1467-2494.2006.00314_2.x |
| Popis: | Levan, a polysaccharide that can be produced by both plants and microorganisms, is a sugar polymer composed of fructose, with beta-2,6 linkages. Here, we have attempted to assess the possible use of levan produced by Zymomonas mobilis as a cosmeceutical ingredient. In service of this goal, we assessed a host of levan's properties, including its moisturizing effects, cell cytotoxicity, cell proliferation effects, and anti-inflammation effects. Levan exhibited a moisturizing effect that was almost exactly the same as that evidenced by hyaluronic acid, as well as a similar cell proliferation effect in human fibroblast and keratinocyte cell lines. Moreover, in our cell proliferation test, which was conducted using bio-artificial skin constructed via 3-dimensional (3-D) culture after the induction of primary skin inflammation with 0.05% sodium lauryl sulfate (SLS), cell viability in the presence of levan (0.01 mg/ml, 0.05 mg/ml) was determined to be higher than cell viability in the absence of levan. In our anti-inflammation test, which was also conducted using 3-D artificial skin, and which involved the measurement of a quantity of secreted interleukin-1alpha (IL-1alpha), a pre-inflammatory mediator induced by SLS, we determined that the quantity of IL-1alpha in the 3-D artificial skin treated with 0.01 mg/ml and 0.05 mg/ml of levan was less than that registered in a skin sample that had been treated only with SLS. In this study, we determined that levan exerted an anti-inflammatory effect against inflammatory reactions to skin irritants, and also that levan exerted a cell-proliferative effect in bio-artificial skin, thereby indicating its potential applicability as a cosmeceutical agent. |
| Databáze: | OpenAIRE |
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