Screening of Environmental DNA Libraries for the Presence of Genes Conferring Lipolytic Activity on Escherichia coli
| Autor: | Rolf Daniel, Ruth A. Schmitz, Anke Henne, Mechthild Bömeke, Gerhard Gottschalk |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Tributyrin
Molecular Sequence Data Genetics and Molecular Biology medicine.disease_cause Applied Microbiology and Biotechnology Genetic analysis law.invention Microbiology 03 medical and health sciences chemistry.chemical_compound law Escherichia coli medicine Amino Acid Sequence Lipase Gene Soil Microbiology Triglycerides Gene Library 030304 developmental biology 0303 health sciences Ecology biology 030306 microbiology Esterases biology.organism_classification Enterobacteriaceae chemistry Recombinant DNA biology.protein Electrophoresis Polyacrylamide Gel Triolein Bacteria Plasmids Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 66:3113-3116 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.66.7.3113-3116.2000 |
| Popis: | Environmental DNA libraries prepared from three different soil samples were screened for genes conferring lipolytic activity on Escherichia coli clones. Screening on triolein agar revealed 1 positive clone out of 730,000 clones, and screening on tributyrin agar revealed 3 positive clones out of 286,000 E. coli clones. Substrate specificity analysis revealed that one recombinant strain harbored a lipase and the other three contained esterases. The genes responsible for the lipolytic activity were identified and characterized. |
| Databáze: | OpenAIRE |
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