Angelica keiskei (Ashitaba) powder and its functional compound xanthoangelol prevent heat stress-induced impairment in sperm density and quality in mouse testes
| Autor: | Yukiko Abe, Atsushi Asano, Hana Ishizaki, Shigeki Yoshida, Shin-ichi Kashiwabara, Hitoshi Miyazaki, Daichi Kokubu, Ryousuke Ooba |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Antioxidant medicine.medical_treatment Ashitaba Heat stress Male infertility Mice 03 medical and health sciences Chalcone 0302 clinical medicine Heat shock protein Internal medicine Testis medicine Animals HSPA1L Angelica 030304 developmental biology 0303 health sciences 030219 obstetrics & reproductive medicine Sperm Count biology Plant Extracts Chemistry Angelika keiskei (Ashitaba) Oligospermia medicine.disease biology.organism_classification Spermatozoa Glutathione synthase Sperm Semen Analysis Endocrinology biology.protein Original Article Antioxidant enzymes Animal Science and Zoology Powders Spermatogenesis Heat-Shock Response |
| Zdroj: | The Journal of Reproduction and Development |
| ISSN: | 0916-8818 |
| Popis: | Recently, gradual decline in human sperm production has become a serious worldwide concern because it leads to increased rates of infertility. Endocrine disrupters, lifestyle changes, and varicocele, all of which elevate testicular temperature, are thought to be the main causes of this decline. The present study aimed to determine whether the dietary phytochemicals Angelica keiskei (Ashitaba) powder (57.5 mg/kg) and its functional component, xanthoangelol (3 mg/kg), can prevent heat stress-induced impairment in sperm density and quality in mice. Sperm parameters were analyzed 28 days after mice exposure to heat. Supplementation with Ashitaba powder completely prevented heat-induced impairment in sperm parameters, including densities of motile sperms and progressive sperms (> 25 μm/sec), and amplitude of lateral head displacement. Xanthoangelol did not exert a complete protective effect; nevertheless, it significantly prevented heat stress-induced reduction in most parameters. Both Ashitaba powder and xanthoangelol elevated the expression of the widely expressed heat shock proteins (HSPs) Hspa1a and Hsp40 and the antioxidant enzyme glutathione synthase in non-stressed testes. Ashitaba powder significantly prevented heat stress-induced reduction in the expression of Hspa1l and Hspa2, which are highly expressed in the testes and critical for fertility. Our results showed that Ashitaba powder and xanthoangelol protected testicular cells from heat stress, probably by elevating the levels of antioxidant enzymes and HSPs. Supplementation with dietary functional phytochemicals may help prevent heat stress-induced male infertility. |
| Databáze: | OpenAIRE |
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