Capsular Switching in Group B Streptococcus CC17 Hypervirulent Clone: A Future Challenge for Polysaccharide Vaccine Development
| Autor: | Agnès Fouet, Claire Poyart, Anne Six, Samuel Bellais, Philippe Glaser, Magalie Longo, Patrick Trieu-Cuot, Nicolas Dmytruk |
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| Přispěvatelé: | Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre national de Référence des Streptocoques (CNR), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Biologie des Bactéries pathogènes à Gram-positif, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Service de Bactériologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], This work was supported by the Fondation pour la Recherche Médicale (grant number DEQ20100318279), ANR (grant number ANR-08-GENM-027–001), INSERM, CNRS, Université Paris Descartes, the Institut Pasteur, and Institut de Veille Sanitaire. S. B. was a recipient of a postdoctoral fellowship from ANR (grant number ANR-08-MIEN-015), the Fondation pour la Recherche Médicale. A. S. was a recipient of a doctoral fellowship from the Ministère de la Recherche et de l'Enseignement supérieur., ANR-08-MIEN-0015,HYPERVIRGBS,Rôle de la protéine Gbs2018C dans l'hypervirulence du clone ST-17 du Streptocoque du groupe B(2008), ANR-08-GENM-0027,Genopop-GBS,Génomique des populations de Streptococcus agalactiae, la diversification des génomes et l'adaptation à l'hôte(2008), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Cochin [AP-HP], Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut Cochin ( UM3 (UMR 8104 / U1016) ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Assistance publique - Hôpitaux de Paris (AP-HP), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Cochin [AP-HP], ANR-08-MIEN-0015,HYPERVIRGBS,Rôle de la protéine Gbs2018C dans l'hypervirulence du clone ST-17 du Streptocoque du groupe B ( 2008 ), ANR-08-GENM-0027,Genopop-GBS,Génomique des populations de Streptococcus agalactiae, la diversification des génomes et l'adaptation à l'hôte ( 2008 ) |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
MESH : France/epidemiology
MESH : Multilocus Sequence Typing [ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology medicine.disease_cause MESH: Genome Bacterial MESH: Streptococcal Infections/microbiology Immunology and Allergy Pathogen MESH : Gene Expression Regulation Bacterial/physiology MESH: Gene Expression Regulation Bacterial/physiology 0303 health sciences education.field_of_study MESH: France/epidemiology Streptococcus Polysaccharides Bacterial MESH : Infant MESH : Adult MESH : Bacterial Capsules/genetics MESH: Infant 3. Good health Infectious Diseases MESH: Multilocus Sequence Typing MESH : Streptococcus agalactiae/pathogenicity France MESH : Streptococcal Infections/microbiology Adult MESH : Genome Bacterial Population Biology Polysaccharide Vaccine MESH: Polysaccharides Bacterial/immunology MESH : Streptococcal Infections/prevention & control Streptococcus agalactiae Microbiology MESH: Bacterial Capsules/metabolism 03 medical and health sciences MESH: Streptococcal Infections/prevention & control MESH : Streptococcus agalactiae/immunology MESH : Streptococcal Infections/epidemiology MESH : Bacterial Capsules/metabolism Streptococcal Infections medicine Pulsed-field gel electrophoresis Humans MESH: Streptococcus agalactiae/immunology Typing Serotyping education Bacterial Capsules 030304 developmental biology 030306 microbiology Infant MESH: Serotyping MESH: Adult Gene Expression Regulation Bacterial bacterial infections and mycoses Virology [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: Streptococcus agalactiae/pathogenicity Multilocus sequence typing bacteria MESH : Polysaccharides Bacterial/immunology MESH: Streptococcal Infections/epidemiology MESH : Serotyping Genome Bacterial MESH: Bacterial Capsules/genetics Multilocus Sequence Typing |
| Zdroj: | Journal of Infectious Diseases Journal of Infectious Diseases, Oxford University Press (OUP), 2012, 206 (11), pp.1745-1752. ⟨10.1093/infdis/jis605⟩ Journal of Infectious Diseases, 2012, 206 (11), pp.1745-1752. ⟨10.1093/infdis/jis605⟩ Journal of Infectious Diseases, Oxford University Press (OUP), 2012, 206 (11), pp.1745-1752. 〈10.1093/infdis/jis605〉 |
| ISSN: | 0022-1899 1537-6613 |
| DOI: | 10.1093/infdis/jis605⟩ |
| Popis: | Background. The capsular polysaccharide (CPS) is an important virulence factor and a vaccine target of the major neonatal pathogen group B Streptococcus (GBS). Population studies revealed no strong correlation between CPS type and multilocus sequence typing (MLST) cluster, with the remarkable exception of the worldwide spread of hypervirulent GBS CC17, which were all until recently CPS type III. Methods. A total of 965 GBS strains from invasive infection isolated in France were CPS typed and the presence of the CC17-specific surface protein encoding gene hvgA gene was investigated. Three hvgA-positive GBS strains screened were surprisingly CPS type IV and thus further characterized by MLST typing, pulsed-field gel electrophoresis (PFGE), and whole genome sequencing. Results. MLST and PFGE demonstrated a capsular switching from CPS type III to IV within the highly homogeneous GBS CC17. Sequence analysis revealed that this capsular switch was due to the exchange of a 35.5-kb DNA fragment containing the entire cps operon. Conclusions. This work shows that GBS CC17 hypervirulent strains have switched one of their main vaccine targets. Thus, continued surveillance of GBS population remains of the utmost importance during clinical trials of conjugate GBS vaccines. Streptococcus agalactiae or group B Streptococcus (GBS) is a gram-positive encapsulated commensal bacterium of the human intestine that is also present in the vagina of 15%–30% of healthy women. In neonates, it may turn into a deadly pathogen and is the |
| Databáze: | OpenAIRE |
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