Increased Expression of CD169 on Blood Monocytes and Its Regulation by Virus and CD8 T Cells in Macaque Models of HIV Infection and AIDS
| Autor: | Chie Sugimoto, Michael M. Kim, Adam R. Filipowicz, Gerard E. Holder, Woong-Ki Kim, Xianhong Liu, Christopher M. McGary, Marcelo J. Kuroda, Hind A. Beydoun, Yanhui Cai |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Sialic Acid Binding Ig-like Lectin 1 Immunology Clinical Sciences Simian Acquired Immunodeficiency Syndrome Spleen Biology CD8-Positive T-Lymphocytes medicine.disease_cause Macaque Virus Monocytes Vaccine Related Plasma biology.animal Virology medicine Cytotoxic T cell Animals 2.1 Biological and endogenous factors 2.2 Factors relating to the physical environment Longitudinal Studies Aetiology Animal Monocyte Prevention Simian immunodeficiency virus Viral Load biology.organism_classification Flow Cytometry Immunohistochemistry Rhesus macaque Disease Models Animal medicine.anatomical_structure Infectious Diseases Good Health and Well Being Disease Models Macaca HIV/AIDS Simian Immunodeficiency Virus Infection Viral load |
| Zdroj: | AIDS research and human retroviruses, vol 31, iss 7 |
| Popis: | Increased expression of CD169 on monocytes has been reported in HIV-1-infected humans. Using rhesus macaque models of HIV infection, we sought to investigate whether simian immunodeficiency virus (SIV) infection upregulates CD169 expression on monocytes/macrophages. We also sought to determine whether CD8 T cells and plasma viral load directly impact the expression of CD169 on monocytes during SIV infection. We longitudinally assessed monocyte expression of CD169 during the course of SIV infection by flow cytometry, and examined the expression of CD169 on macrophages by immunohistochemistry in the spleen and lymph nodes of uninfected and infected macaques. CD169 expression on monocytes was substantially upregulated as early as 4 days during the hyperacute phase and peaked by 5-15 days after infection. After a transient decrease following the peak, its expression continued to increase during progression to AIDS. Monocyte CD169 expression was directly associated with plasma viral loads. To determine the contribution of CD8(+) T lymphocytes and virus to the control of monocyte CD169 expression, we used experimental CD8(+) lymphocyte depletion and antiretroviral therapy (ART) in SIV-infected macaques. Rapid depletion of CD8 T cells during acute infection of rhesus macaques induced an abrupt increase in CD169 expression. Importantly, levels of CD169 expression plummeted following initiation of ART and rebounded upon cessation of therapy. Taken together, our data reveal independent roles for virus and CD8(+) T lymphocytes in controlling monocyte CD169 expression, which may be an important link in further investigating the host response to viral infection. |
| Databáze: | OpenAIRE |
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