In vitro synthesis of β2-microglobulin by human fetal tissues
| Autor: | Jan Cejka, Karel Kithier, J. Belamaric, Ward D. Peterson |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Male
Beta-Globulins Fetal tissue Thymus Gland In Vitro Techniques Biology Kidney Fetus Fetal cell Testis Humans Carbon Radioisotopes Molecular Biology chemistry.chemical_classification Beta-2 microglobulin Immune Sera Infant Newborn Cell Biology Fetal Blood Ouchterlony double immunodiffusion Molecular biology In vitro Amino acid Liver chemistry Cell culture Human fetal Developmental Biology |
| Zdroj: | Developmental Biology. 43:200-203 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/0012-1606(75)90141-4 |
| Popis: | Human fetal tissues were cultured in the presence of 14C-labeled amino acids and the culture fluids analyzed by radioimmunoelectrophoresis for the presence of radioactive β2-microglobulin. Synthesis of the protein was demonstrated in all the tissues studied. Using the Ouchterlony method, β2-microglobulin was also detected in culture media from serially transferred cell cultures of fetal tissues and established fetal cell strains. |
| Databáze: | OpenAIRE |
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