Preparation and Testing of Impedance-based Fluidic Biochips with RTgill-W1 Cells for Rapid Evaluation of Drinking Water Samples for Toxicity
| Autor: | Michael K. McAleer, Alex Greis, Michael W. Mayo, Linda M. Brennan, William H. van der Schalie, Mark W. Widder |
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| Rok vydání: | 2016 |
| Předmět: |
Gills
0301 basic medicine General Chemical Engineering biochip Cold storage Nanotechnology Portable water purification Biosensing Techniques sensors General Biochemistry Genetics and Molecular Biology Cell Line 03 medical and health sciences monolayer Toxicity Tests Electric Impedance Animals Humans Fluidics Biochip ECIS General Immunology and Microbiology Drinking Water General Neuroscience Epithelial Cells water toxicity Contamination field-portable 030104 developmental biology Oncorhynchus mykiss impedance Toxicity Environmental science Water treatment Issue 109 Water quality Fish cells Environmental Sciences Water Pollutants Chemical |
| Zdroj: | Journal of Visualized Experiments : JoVE |
| ISSN: | 1940-087X |
| DOI: | 10.3791/53555 |
| Popis: | This manuscript describes how to prepare fluidic biochips with Rainbow trout gill epithelial (RTgill-W1) cells for use in a field portable water toxicity sensor. A monolayer of RTgill-W1 cells forms on the sensing electrodes enclosed within the biochips. The biochips are then used for testing in a field portable electric cell-substrate impedance sensing (ECIS) device designed for rapid toxicity testing of drinking water. The manuscript further describes how to run a toxicity test using the prepared biochips. A control water sample and the test water sample are mixed with pre-measured powdered media and injected into separate channels of the biochip. Impedance readings from the sensing electrodes in each of the biochip channels are measured and compared by an automated statistical software program. The screen on the ECIS instrument will indicate either "Contamination Detected" or "No Contamination Detected" within an hour of sample injection. Advantages are ease of use and rapid response to a broad spectrum of inorganic and organic chemicals at concentrations that are relevant to human health concerns, as well as the long-term stability of stored biochips in a ready state for testing. Limitations are the requirement for cold storage of the biochips and limited sensitivity to cholinesterase-inhibiting pesticides. Applications for this toxicity detector are for rapid field-portable testing of drinking water supplies by Army Preventative Medicine personnel or for use at municipal water treatment facilities. |
| Databáze: | OpenAIRE |
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