Isolation of the pellicle ofToxoplasma gondii (Protozoa, Coccidia): Characterization by electron microscopy and protein composition
| Autor: | Y Gallois, G. Tronchin, R. Robert, G. Mauras, F Foussard |
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| Rok vydání: | 1990 |
| Předmět: |
Gel electrophoresis
General Veterinary biology Cell Membrane Protozoan Proteins Membrane Proteins Toxoplasma gondii General Medicine biology.organism_classification Microspheres Microscopy Electron Electrophoresis Infectious Diseases Coccidia Affinity chromatography Biochemistry Insect Science Centrifugation Density Gradient Ultrastructure Animals Protozoa Electrophoresis Polyacrylamide Gel Parasitology Centrifugation Toxoplasma |
| Zdroj: | Parasitology Research. 76:563-565 |
| ISSN: | 1432-1955 0044-3255 |
| DOI: | 10.1007/bf00932561 |
| Popis: | Two methods were compared for the isolation of the Toxoplasma gondii pellicle: centrifugation using a discontinuous sucrose gradient and affinity purification by microspheres activated with protein A. Electron microscopy showed that these two means of purification gave similar results, although better yields were obtained with the former than with the latter. Proteins of membrane fractions isolated using sucrose gradient were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Bands in the molecular-weight regions of 19, 22, 35, and 79 kDa appeared to be associated with the T. gondii pellicle. |
| Databáze: | OpenAIRE |
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