Establishment of novel technical methods for evaluating brain edema and lesion volume in stroked rats: A standardization of measurement procedures
| Autor: | Ilan Shelef, Dmitry Frank, Vladislav Zvenigorodsky, Israel Melamed, Matthew Boyko, Honore N Shiyntum, Ruslan Kutz, Alexander Zlotnik, Julia Grinshpun, Evgeni Brotfain |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Lesion volume Brain Edema Rats Sprague-Dawley 03 medical and health sciences High morbidity 0302 clinical medicine Image Processing Computer-Assisted Medicine Animals Molecular Biology Stroke 3 Tesla Magnetic Resonance Imaging medicine.diagnostic_test business.industry Brain edema General Neuroscience Follow up studies Brain Reproducibility of Results Magnetic resonance imaging Gold standard (test) equipment and supplies medicine.disease Magnetic Resonance Imaging Rats 030104 developmental biology Diffusion Magnetic Resonance Imaging Brain Injuries Neurology (clinical) Radiology business human activities 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Brain research. 1718 |
| ISSN: | 1872-6240 |
| Popis: | Stroke plays a role in high morbidity and mortality. Deciphering its mechanisms and pathophysiology is critical for the creation of new drugs and therapies. Most of the previous animal models of stroke, aimed at identifying the extent and location of brain injury following stroke, require animal sacrifice, which, besides ethical considerations, also negates the ability for follow up studies with the same rats. Because of these failures, the use of clinical magnetic resonance scanners for evaluating small animal models has been increasing. Magnetic resonance imaging scanners used particularly for small-bore animals are eligible for use in high-resolution magnetic resonance imaging of rodent brains. However, high costs and scarcity factor heavily in the rare availability of these scanners. In our investigation, we sought to establish a unitary magnetic resonance imaging protocol for stroke assessment in rats. We made use of a 3-Tesla magnetic resonance imaging clinical scanner, as well as another clinical equipment, with the purpose of increasing its reproducibility. The results of inquest validated a new magnetic resonance imaging protocol, comparing a magnetic resonance imaging-measured infarcted zone to the “gold standard” of histological examination. We carried out the experimental procedure on a 3 Tesla magnetic resonance imaging clinical scanner using a conventional eight-channel receive-only coil. The two methods produced remarkable quantitative and qualitative correlations between them. Conclusively, we showed the clinical magnetic resonance imaging scanner to be a high-precision and sensitive image analysis instrument for evaluating both the infarct zone and the brain edema in a stroke experimental rat model. |
| Databáze: | OpenAIRE |
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