Cellular composition of primary cultures of human granulosa-lutein cells and the effect of cytokines on cell proliferation
| Autor: | V. Pascoe, Robert J. Norman, Mats Brännström, Ling Jia Wang |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Interleukin 2
Cell type Necrosis Time Factors medicine.drug_class Monoclonal antibody Monocytes Endocrinology Antigen Luteal Cells Genetics medicine Humans Lymphocytes Granulosa Lutein Cell Molecular Biology Cells Cultured CD40 biology Tumor Necrosis Factor-alpha Macrophages Molecular biology Reproductive Medicine Immunology biology.protein Interleukin-2 Animal Science and Zoology Tumor necrosis factor alpha Female medicine.symptom Cell Division Developmental Biology Biotechnology medicine.drug |
| Zdroj: | Reproduction, fertility, and development. 7(1) |
| ISSN: | 1031-3613 |
| Popis: | The cellular composition of cells collected from the follicular fluid obtained during the IVF procedure and cultured in vitro was examined, as well as the effects of two cytokines, interleukin-2 (IL-2) and tumour necrosis factor-alpha (TNF alpha), on the proliferation of individual cell types. After 48 h in culture, most of the cells were granulosa-lutein cells exhibiting positive staining against 3-beta-hydroxysteroid dehydrogenase (3 beta-HSD). About one-third of the total cell population stained positive with monoclonal antibodies against specific antigen sites on lymphohaemopoietic cells, including B-lymphocytes, T-lymphocytes, natural killer cells, monocytes and macrophages. During this 48-h period, IL-2 and TNF alpha significantly (P < 0.05) increased the proportion of leukocyte common antigen (LCA)-positive cells and monocytes. At the end of 144 h in culture, although LCA-positive cells and monocytes were still present, there were fewer. The main targets for the proliferative effects of IL-2 and TNF alpha in this culture system during the first 48 h are leukocytes rather than steroid-producing cells. Thus, any observed effects from the addition of cytokines in this system may be due to indirect effects of cytokine-activated leukocytes on granulosa-lutein cells. |
| Databáze: | OpenAIRE |
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