Standardization of an enzyme immunoassay for human antibody to Haemophilus ducreyi
| Autor: | J O Ndinya-Achola, A R Ronald, L G Filion, C E Thompson, D. W. Cameron, Peter Piot, FA Plummer, Marc Desjardins |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1992 |
| Předmět: |
Microbiology (medical)
Sexually transmitted disease Male Biology urologic and male genital diseases Serology Chancroid Haemophilus ducreyi Immunoenzyme Techniques HIV Seropositivity medicine Humans medicine.diagnostic_test Reference Standards biology.organism_classification medicine.disease bacterial infections and mycoses Virology Antibodies Bacterial female genital diseases and pregnancy complications Immunoglobulin M Evaluation Studies as Topic Immunoassay Immunoglobulin G Immunology Coinfection biology.protein Female Bacterial antigen Research Article |
| Zdroj: | Scopus-Elsevier |
| Popis: | We standardized a serologic enzyme immunoassay (EIA) for human immunoglobulin G and M antibodies against Haemophilus ducreyi. We evaluated the performance of this test with respect to the time from acute chancroid and coinfection with human immunodeficiency virus (HIV). Antibody to a crude, soluble bacterial antigen of one H. ducreyi strain was detected in a panel of serum samples from clinically and microbiologically confirmed cases of chancroid and from controls. Test interpretation was standardized for optimal sensitivity and specificity. Performance of the EIA was enhanced in the period of early convalescence from acute primary chancroid and was not diminished in the presence of HIV coinfection. The EIA performed adequately as a serologic screening test for field evaluation and epidemiologic application in conjunction with sexually transmitted disease and HIV detection and control efforts. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|