Selective detection of 2-nitrobenzenesulfenyl-labeled peptides by matrix-assisted laser desorption/ionization-time of flight mass spectrometry using a novel matrix
| Autor: | Susumu Tsunasawa, Osamu Nishimura, Chikako Toda, Shunsuke Izumi, Noriyuki Ojima, Ei-ichi Matsuo, Makoto Watanabe, Koichi Tanaka |
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| Rok vydání: | 2006 |
| Předmět: |
chemistry.chemical_classification
Chromatography Staining and Labeling Molecular Sequence Data Tryptophan food and beverages Peptide Mass spectrometry Biochemistry Matrix (chemical analysis) Nitrobenzene chemistry.chemical_compound Matrix-assisted laser desorption/ionization chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization embryonic structures Amino Acid Sequence Peptides Selectivity Molecular Biology Quantitative analysis (chemistry) Nitrobenzenes |
| Zdroj: | PROTEOMICS. 6:2042-2049 |
| ISSN: | 1615-9861 1615-9853 |
| DOI: | 10.1002/pmic.200500575 |
| Popis: | The 2-nitrobenzenesulfenyl (NBS) method, which is useful for quantitative proteome analysis, is based on stable isotope labeling of tryptophan residues with NBS chloride ((12)C(6)-NBSCl or (13)C(6)-NBSCl). We found that 3-hydroxy-4-nitrobenzoic acid (3H4NBA) is a more suitable matrix than 2,5-dihydroxybenzoic acid (DHB) for detecting NBS-labeled peptides by MALDI-quadrupole IT (QIT)-TOF MS . Furthermore, NBS-labeled peptides were selectively ionized and detected in a mixture of NBS-labeled and unlabeled peptides. Labeled paired peaks were easily detected without enrichment, nonpaired labeled peaks were clearly distinguished from unlabeled contaminating peptides, and nitrotyrosine-containing peptides were also selectively detected on the 3H4NBA matrix, while by-product-peaks arising from nitrobenzene moieties were suppressed. The use of 3H4NBA as a comatrix with CHCA improved the sensitivity of detection while substantially retaining the selectivity of 3H4NBA. The 3H4NBA matrix offers great advantages in terms of simplicity, sensitivity, and usability when used for the NBS method and for MALDI-TOF MS analysis applied to compounds having a nitrobenzene ring. |
| Databáze: | OpenAIRE |
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